1887

Abstract

A previously undescribed, slowly growing, scotochromogenic mycobacterium was isolated from a patient with symptomatic pulmonary infection during sequence-based identification of Korean clinical isolates. Phenetic characteristics of this strain were generally similar to those of and . However, some phenetic characteristics differentiated it from these two species. Its 16S rRNA gene sequences were unique and phylogenetic analysis based on 16S rRNA gene sequences placed the organism in the slowly growing group close to and . Its unique mycolic acid profiles and the results of phylogenetic analysis based on two independent alternative chronometer molecules, and , confirmed the taxonomic status of this strain as representing a novel species. These data support the conclusion that this strain represents a novel mycobacterial species, for which the name sp. nov. is proposed. The type strain is strain 03-19 (=DSM 44998=KCTC 19146).

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2007-03-01
2019-12-13
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References

  1. Butler, W. R., Thibert, L. & Kilburn, J. O. ( 1992; ). Identification of Mycobacterium avium complex strains and some similar species by high-performance liquid chromatography. J Clin Microbiol 30, 2698–2704.
    [Google Scholar]
  2. CDC ( 1996; ). Standardized method for HPLC identification of mycobacteria. Atlanta, GA: Centers for Disease Control and Prevention. http://www.cdc.gov/ncidod/publications/hplc.pdf
  3. CDC ( 1999; ). Mycolic acid pattern standards for HPLC identification of mycobacteria. Atlanta, GA: Centers for Disease Control and Prevention. http://www.cdc.gov/nchstp/tb/Laboratory_Services/maps_tagged.pdf
  4. Duffey, P. S., Guthertz, L. S. & Evans, G. C. ( 1996; ). Improved rapid identification of mycobacteria by combining solid-phase extraction with high-performance liquid chromatography analysis of BACTEC cultures. J Clin Microbiol 34, 1939–1943.
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  7. Floyd, M. M., Guthertz, L. S., Silcox, V. A., Duffey, P. S., Jang, Y., Desmond, E. P., Crawford, J. T. & Butler, W. R. ( 1996; ). Characterization of an SAV organism and proposal of Mycobacterium triplex sp. nov. J Clin Microbiol 34, 2963–2967.
    [Google Scholar]
  8. Goodfellow, M. & Magee, J. G. ( 1998; ). Taxonomy of mycobacteria. In Mycobacteria, vol. 1, Basic Aspects, pp. 1–71. Edited by P. R. J. Gangadharam & P. A. Jenkins. New York & London: Chapman & Hall.
  9. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  10. Kent, P. T. & Kubica, G. P. ( 1985; ). Public Health Mycobacteriology. Guide for the level III Laboratory. US Department of Health and Human Service publication. Atlanta, GA: Centers for Disease Control and Prevention.
  11. Kim, B. J., Lee, S. H., Lyu, M. A., Kim, S. J., Bai, G. H., Chae, G. T., Kim, E. C., Cha, C. Y. & Kook, Y. H. ( 1999; ). Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB). J Clin Microbiol 37, 1714–1720.
    [Google Scholar]
  12. Kim, H., Kim, S.-H., Shim, T.-S., Kim, M.-n., Bai, G.-H., Park, Y.-G., Lee, S.-H., Chae, G.-T., Cha, C.-Y. & other authors ( 2005; ). Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65). Int J Syst Evol Microbiol 55, 1649–1656.[CrossRef]
    [Google Scholar]
  13. Kirschner, P., Springer, B., Vogel, U., Meier, A., Wrede, A., Kiekenbeck, M., Bange, F. C. & Bottger, E. C. ( 1993; ). Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J Clin Microbiol 31, 2882–2889.
    [Google Scholar]
  14. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Tempe, AZ: Arizona State University.
  15. Roth, A., Fischer, M., Hamid, M. E., Michalke, S., Ludwig, W. & Mauch, H. ( 1998; ). Differentiation of phylogenetically related slowly growing mycobacteria based on 16S-23S rRNA gene internal transcribed spacer sequences. J Clin Microbiol 36, 139–147.
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Springer, B., Stockman, L., Teschner, K., Roberts, G. D. & Bottger, E. C. ( 1996; ). Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. J Clin Microbiol 34, 296–303.
    [Google Scholar]
  18. Stahl, D. A. & Urbance, J. W. ( 1990; ). The division between fast- and slow-growing species corresponds to natural relationships among the mycobacteria. J Bacteriol 172, 116–124.
    [Google Scholar]
  19. Stone, B. B., Nietupski, R. M., Breton, G. L. & Weisburg, W. G. ( 1995; ). Comparison of Mycobacterium 23S rRNA sequences by high-temperature reverse transcription and PCR. Int J Syst Bacteriol 45, 811–819.[CrossRef]
    [Google Scholar]
  20. Wayne, L. G. & Kubica, G. P. ( 1986; ). The mycobacteria. In Bergey's Manual of Determinative Bacteriology, 9th edn, pp. 1435–1457. Edited by R. E. Buchanan & N. E. Gibbons. Baltimore: Williams & Wilkins.
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[PDF files of Supplementary Figs S1 and S2](35 KB)

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Alignments of partial gene sequences (603 bp) (a) and partial gene sequences (306 bp) (b) from 03-19 and genotypically similar reference strains. [PDF](17 KB)

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