1887

Abstract

The ammonia-producing bacteria B55, CA73, SA69 and SA72 were isolated from the waterweeds (B55) and (CA73, SA69 and SA72) grown in highly acidic swamps (pH 2–4) in actual acid sulfate soil areas of Vietnam. The isolates were Gram-positive, irregular rod-shaped, non-spore-forming bacteria. On the basis of 16S rRNA gene sequence similarity, strain B55 was shown to belong to the genus of the class . Chemotaxonomic data (MK-9 as major isoprenoid quinone, -ornithine as cell-wall diamino acid, acetyl as the acyl type of peptidoglycan) supported the affiliation of all four strains to this genus. Although their 16S rRNA gene sequence similarity was 99 % to species with validly published names within the genus, they formed a group that was distinct in the phylogenetic tree, and DNA–DNA relatedness values to these established species were less than 10 %. The results of physiological and biochemical tests and major fatty acids (cyclohexyl-C, anteiso-C and cyclohexyl-C) allowed phenotypic differentiation of these strains from the species of with validly published names. Therefore, strains B55, CA73, SA69 and SA72 represent a novel species, for which the name sp. nov. is proposed. The type strain is B55 (=NBRC 101786=VTCC D6-11=JCM 14609).

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2007-07-01
2024-03-29
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References

  1. Behrendt U., Ulrich A., Schumann P., Naumann D., Suzuki K. 2002; Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of Subtercola pratensis sp.nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov.,sp. nov. Int J Syst Evol Microbiol 52:1441–1454 [CrossRef]
    [Google Scholar]
  2. Collins M. D., Jones D. 1983; Reclassification of Corynebacterium flaccumfaciens , Corynebacterium betae , Corynebacterium oortii and Corynebacterium poinsettiae in the genus Curtobacterium , as Curtobacterium flaccumfaciens comb. nov. J Gen Microbiol 129:3545–3548
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Takeuchi N., Yamamoto H., Liu S. L., Miura H., Matsui K., Yabuuchi E. 1988; Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells. J Clin Microbiol 26:1708–1713
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 2005 phylip (Phylogeny Inference Package) version 3.65. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  7. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  8. Iizuka H., Komagata K. 1965; Microbiological studies on petroleum and natural gas. III. Determination of Brevibacterium , Arthrobacter , Micrococcus , Sarcina , Alcaligenes , and Achromobacter isolated from oil-brines in Japan. J Gen Appl Microbiol 11:1–14
    [Google Scholar]
  9. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  10. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  11. Komagata K., Iizuka H. 1964; Studies on the microorganisms of cereal grains. Part VII. New species of Brevibacterium isolated from rice. J Agric Chem Soc Jpn 38:496–502
    [Google Scholar]
  12. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  13. Nishiuchi Y., Baba T., Hotta H. H., Yano I. 1999; Mycolic acid analysis in Nocardia species: the mycolic acid compositions of Nocardia asteroides , N. farcinica , and N. nova . J Microbiol Methods 37:111–122 [CrossRef]
    [Google Scholar]
  14. Ryu E. 1938; On the Gram-differentiation of bacteria by the simplest method. J Jpn Soc Vet Sci 17:31
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  17. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  18. Stackebrandt E., Goebel B. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  19. Suzuki K., Saito K., Kawaguchi A., Okuda S., Komagata K. 1981; Occurrence of ω -cyclohexyl fatty acids in Curtobacterium pusillum strains. J Gen Appl Microbiol 27:261–266 [CrossRef]
    [Google Scholar]
  20. Tamura T., Hatano K. 2001; Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘ Actinoplanes aurantiacus ’ to Cryptosporangium minutisporangium comb.nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51:2119–2125 [CrossRef]
    [Google Scholar]
  21. Tamura T., Nakagaito Y., Nishii T., Hasegawa T., Stackebrandt E., Yokota A. 1994; A new genus of the order Actinomycetales , Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb.nov. and Couchioplanescaeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44:193–203 [CrossRef]
    [Google Scholar]
  22. Tamura T., Hayakawa M., Hatano K. 1999; Sporichthya brevicatena sp. nov. Int J Syst Bacteriol 49:1779–1784 [CrossRef]
    [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  24. Uchida K., Kudo T., Suzuki K., Nakase T. 1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56 [CrossRef]
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  26. Yamada K., Komagata K. 1972; Taxonomic studies on coryneform bacteria. V. Classification of coryneform bacteria. J Gen Appl Microbiol 18:417–431 [CrossRef]
    [Google Scholar]
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