1887

Abstract

The ammonia-producing bacteria B55, CA73, SA69 and SA72 were isolated from the waterweeds (B55) and (CA73, SA69 and SA72) grown in highly acidic swamps (pH 2–4) in actual acid sulfate soil areas of Vietnam. The isolates were Gram-positive, irregular rod-shaped, non-spore-forming bacteria. On the basis of 16S rRNA gene sequence similarity, strain B55 was shown to belong to the genus of the class . Chemotaxonomic data (MK-9 as major isoprenoid quinone, -ornithine as cell-wall diamino acid, acetyl as the acyl type of peptidoglycan) supported the affiliation of all four strains to this genus. Although their 16S rRNA gene sequence similarity was 99 % to species with validly published names within the genus, they formed a group that was distinct in the phylogenetic tree, and DNA–DNA relatedness values to these established species were less than 10 %. The results of physiological and biochemical tests and major fatty acids (cyclohexyl-C, anteiso-C and cyclohexyl-C) allowed phenotypic differentiation of these strains from the species of with validly published names. Therefore, strains B55, CA73, SA69 and SA72 represent a novel species, for which the name sp. nov. is proposed. The type strain is B55 (=NBRC 101786=VTCC D6-11=JCM 14609).

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2007-07-01
2019-10-18
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Scanning electron micrograph of cells of B55 . Cells were grown in 1/10 TS medium (pH 4.1) at 28 °C for 3 days. After critical-point drying, specimens were sputter-coated with gold palladium and observed with a scanning electron microscope (S-3500N; Hitachi). Bar, 2 µm.

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Cellular fatty acid composition of strains of sp. nov. and closely related type strains. [PDF](16 KB)

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