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Abstract

A non-motile, Gram-negative, boron-tolerant and alkaliphilic bacterium was isolated from soil of the Hisarcik area in the Kutahya Province of Turkey that was naturally high in boron minerals. The novel isolate, designated T-22, formed rod-shaped cells, was catalase- and oxidase-positive and tolerated up to 300 mM boron. The strain also survived on agar medium containing up to 3 % (w/v) NaCl. The pH range for growth of this strain was 6.5–10.0 (optimum pH 8.0–9.0) and the temperature range was 16–37 °C (optimum 28–30 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed a clear affiliation with the genus , with 97.4 % sequence similarity to AC-74, which was the highest similarity among cultivated bacteria. The DNA–DNA relatedness with AC-74 was 28.3 %. The major respiratory quinone system was MK-7 and the predominant cellular fatty acids were iso-C, iso-C 9, iso-C 3-OH and summed feature 3 (iso-C 2-OH and/or iso-C 7). The DNA G+C content was 42.5 mol%. Based on the phylogenetic analysis and physiological, chemotaxonomic and genetic data, we concluded that strain T-22 should be classified in the genus , and we propose the name sp. nov. for this novel species. The type strain is strain T-22 (=DSM 17298=NBRC 101277=ATCC BAA-1189).

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2007-05-01
2019-12-06
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vol. , part 5, pp. 986 - 992

Phylogenetic trees showing the inter-relationships of strain T-22 and its closest relatives inferred from 16S rRNA gene sequences, constructed using the maximum-likelihood algorithm (Fig. S1) and the maximum-parsimony algorithm (Fig. S2). [PDF](22 KB)



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