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Abstract

A Gram-negative, non-motile, rod-shaped bacterial strain, DS-57, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated using a polyphasic approach. It grew optimally at 25 °C and in trypticase soy broth without NaCl and trypticase soy broth with 0.5 % NaCl. Strain DS-57 contained MK-7 as the predominant menaquinone and iso-C, C 7 and/or iso-C 2-OH and iso-C 3-OH as the major fatty acids. The DNA G+C content was 39.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DS-57 was most closely related to the genus of the family , clustering coherently with , and . Strain DS-57 exhibited 16S rRNA gene sequence similarity values of 99.2, 97.9 and 97.2 % with respect to the type strains of . , . and . , respectively, and values less than 95.6 % with respect to the type strains of other species. Strain DS-57 exhibited levels of DNA–DNA relatedness of 45, 17 and 15 % with respect to the type strains of . , . and . , respectively. Differential phenotypic properties, together with the phylogenetic distinctiveness and the DNA–DNA relatedness data, were sufficient to allow the classification of strain DS-57 as a species that is separate from recognized species. On the basis of phenotypic properties and phylogenetic distinctiveness, therefore, strain DS-57 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DS-57 (=KCTC 12762=DSM 17933).

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2007-11-01
2019-10-14
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vol. , part 11, pp. 2462-2466

Cellular fatty acid content of strain DS-57 and phylogenetically related species. [PDF](24 KB)



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