A bacterial strain (E4FC31T) isolated from treated municipal wastewater was characterized phenotypically and phylogenetically. Cells were Gram-negative, curved rods with a polar flagellum. The isolate was catalase-, oxidase- and arginine dihydrolase-positive, and able to grow between 15 and 45 °C and between pH 5.5 and 9.0. The predominant fatty acids were C16 : 1/iso-C15 : 0 2-OH and C16 : 0, the major respiratory quinone was ubiquinone 8 and the G+C content of the genomic DNA was 63 mol%. 16S rRNA gene sequence analysis indicated that strain E4FC31T belonged to the class Betaproteobacteria and was a member of the family Neisseriaceae. Its closest phylogenetic neighbours were Aquitalea magnusonii and Chromobacterium violaceum (<94 % 16S rRNA gene sequence similarity). Phylogenetic analysis and phenotypic characteristics of strain E4FC31T suggest that it represents a novel species of a new genus, for which the name Gulbenkiania mobilis gen. nov., sp. nov. is proposed. The type strain of Gulbenkiania mobilis is E4FC31T (=DSM 18507T=LMG 23770T).
BarreirosL.,
NogalesB.,
ManaiaC. M.,
FerreiraA. C. S.,
PieperD. H.,
ReisM. A.,
NunesO. C.2003; A novel pathway for mineralization of the thiocarbamate herbicide molinate by a defined bacterial mixed culture. Environ Microbiol 5:944–953[CrossRef]
CashionP.,
Holder-FranklinM. A.,
McCullyJ.,
FranklinM.1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466[CrossRef]
ChernL.-L.,
StackebrandtE.,
LeeS.-F.,
LeeF.-L.,
ChenJ.-K.,
FuH.-M.2004; Chitinibacter tainanensis gen. nov., sp. nov. a chitin-degrading aerobe from soil in Taiwan. Int J Syst Evol Microbiol 54:1387–1391[CrossRef]
Comité de l'Antibiogramme de la Société Française de Microbiologie1998; Communiqué du Comité de l'Antibiogramme de la Société Française de Microbiologie. Bull Soc Fr Microbiol 13:243–258
Ferreira da SilvaM.,
TiagoI.,
VeríssimoA.,
BoaventuraA. R.,
NunesO. C.,
ManaiaC. M.2006; Antibiotic resistance of enterococci and related bacteria in an urban wastewater treatment plant. FEMS Microbiol Ecol 55:322–329[CrossRef]
GillisM.,
LoganN. A.2005; Genus IV. Chromobacterium Bergonzini 1881, 153AL. In Bergey's Manual of Systematic Bacteriology
. , 2nd edn. vol. 2, part C pp 824–827 Edited by
BrennerD. J.,
KriegN. R.,
StaleyJ. T.,
GarrityG. M.
New York: Springer;
GrimesD. J.,
WoeseC. R.,
MacDonellM. T.,
ColwellR. R.1997; Systematic study of the genus Vogesella gen. nov. and its type species, Vogesella indigofera comb. nov. Int J Syst Bacteriol 47:19–27[CrossRef]
KuykendallL. D.,
RoyM. A.,
O'NeillJ. J.,
DevineT. E.1988; Fatty acids, antibiotic resistance and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum
. Int J Syst Bacteriol 38:358–361[CrossRef]
LauH.,
FarynaJ.,
TriplettE. W.2006; Aquitalea magnusonii gen. nov., sp. nov., a novel Gram-negative bacterium isolated from a humic lake. Int J Syst Evol Microbiol 56:867–871[CrossRef]
ManaiaC. M.,
NunesO. C.,
NogalesB.2003; Caenibacterium thermophilum gen. nov., sp. nov. isolated from a thermophilic aerobic digester of municipal sludge. Int J Syst Evol Microbiol 53:1375–1382[CrossRef]
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167[CrossRef]
MurrayR. G. E.,
DoetschR. N.,
RobinowF.1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp 21–41 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
PatureauD.,
GodonJ.-J.,
DabertP.,
BouchezT.,
BernetN.,
DelgenesJ. P.,
MolettaR.1998; Microvirgula aerodenitrificans gen. nov., sp. nov. a new Gram-negative bacterium exhibiting co-respiration of oxygen and nitrogen oxides up to oxygen-saturated conditions. Int J Syst Bacteriol 48:775–782[CrossRef]
RaineyF. A.,
Ward-RaineyN.,
KroppenstedtR. M.,
StackebrandtE.1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092[CrossRef]
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
TiagoI.,
TeixeiraI.,
SilvaS.,
ChungP.,
VeríssimoA.,
ManaiaC. M.2004; Metabolic and genetic diversity of mesophilic and thermophilic bacteria isolated from composted municipal sludge on poly- ϵ -caprolactones. Curr Microbiol 49:407–414[CrossRef]
TønjumT.2005; Family I. Neisseriaceae Prèvot 1933, 119AL emend. Dewhirst, Paster and Bright 1989, 265. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 2 part C Edited by
BrennerD. J.,
KriegN. R.,
StaleyJ. T.,
GarrityG. M.
pp 775–776 New York: Springer;
VelaA. I.,
CollinsM. D.,
LawsonP. A.,
GarcíaN.,
DomínguezL.,
Fernández-GarayzábalJ. F.2005; Uruburuella suis gen. nov., sp. nov., isolated from clinical specimens of pigs. Int J Syst Evol Microbiol 55:643–647[CrossRef]
YuenK.-Y.,
WooP. C. Y.,
TengJ. L. L.,
LeungK.-W.,
WongM. K. M.,
LauS. K. P.2001; Laribacter hongkongensis gen. nov., sp. nov., a novel Gram-negative bacterium isolated from a cirrhotic patient with bacteremia and empyema. J Clin Microbiol 39:4227–4232[CrossRef]