sp. nov., the host of ‘Free

Abstract

A novel chemolithoautotrophic and hyperthermophilic member of the genus was isolated from a submarine hydrothermal system at the Kolbeinsey Ridge, to the north of Iceland. The new isolate showed high similarity to the two species described to date, and , in its physiological properties as well as in its unique cell architecture. However, phylogenetic analysis and investigations on the protein composition of the outer membrane demonstrated that the new isolate was clearly distinct from and . Furthermore, it is the only organism known so far which is able to serve as a host for ‘’, the only cultivated member of the ‘’. Therefore, the new isolate represents a novel species of the genus , which we name sp. nov. (type strain KIN4/I=DSM 18386=JCM 14125).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64721-0
2007-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/4/803.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64721-0&mimeType=html&fmt=ahah

References

  1. Balch W. E., Wolfe R. S. 1976; New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl Environ Microbiol 32:781–791
    [Google Scholar]
  2. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. 1981; Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli . J Mol Biol 148:107–127 [CrossRef]
    [Google Scholar]
  3. Burggraf S., Huber H., Stetter K. O. 1997; Reclassification of the crenarchaeal orders and families in accordance with 16S rRNA sequence data. Int J Syst Bacteriol 47:657–660 [CrossRef]
    [Google Scholar]
  4. Burghardt T., Näther D. J., Junglas B., Huber H., Rachel R. 2007; The dominating outer membrane protein of the hyperthermophilic Archaeum Ignicoccus hospitalis : a novel pore-forming complex. Mol Microbiol 63:166–176 [CrossRef]
    [Google Scholar]
  5. Eder W., Ludwig W., Huber R. 1999; Novel 16S rRNA gene sequences retrieved from highly saline brine sediments of Kebrit Deep, Red Sea. Arch Microbiol 172:213–218 [CrossRef]
    [Google Scholar]
  6. Fricke H., Giere O., Stetter K., Alfredsson G. A., Kristjansson J. K., Stoffers P., Svavarsson J. 1989; Hydrothermal vent communities at the shallow subpolar Mid-Atlantic ridge. Mar Biol 102:425–429 [CrossRef]
    [Google Scholar]
  7. Huber H. 2005; Crenarchaeota. In Encyclopedia of Life Sciences Chichester: Wiley; http://www.els.net
    [Google Scholar]
  8. Huber H., Stetter K. O. 2001; Order II. Desulfurococcales ord. nov. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  179–180 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  9. Huber R., Burggraf S., Mayer T., Barns S., Rossnagel P., Stetter K. O. 1995; Isolation of a hyperthermophilic archaeum predicted by in situ RNA analysis. Nature 376:57–58 [CrossRef]
    [Google Scholar]
  10. Huber H., Burggraf S., Mayer T., Wyschkony I., Rachel R., Stetter K. O. 2000; Ignicoccus gen. nov., a novel genus of hyperthermophilic, chemolithoautotrophic Archaea , represented by two new species, Ignicoccus islandicus sp.nov. and Ignicoccus pacificus sp. nov. . Int J Syst Evol Microbiol 50, 2093–2100 [CrossRef]
  11. Huber H., Hohn M. J., Rachel R., Fuchs T., Wimmer V. C., Stetter K. O. 2002; A new phylum of Archaea represented by a nanosized hyperthermophilic symbiont. Nature 417:63–67 [CrossRef]
    [Google Scholar]
  12. Huber H., Hohn M. J., Rachel R., Stetter K. O. 2006 Nanoarchaeota . In The Prokaryotes: a Handbook on the Biology of Bacteria , 3rd edn, vol. 3, Archaea. Bacteria: Firmicutes, Actinomycetes pp  274–280 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  13. Jahn U., Summons R., Sturt H., Grosjean E., Huber H. 2004; Composition of the lipids of Nanoarchaeum equitans and their origin from its host Ignicoccus sp. strain KIN4/I. Arch Microbiol 182:404–413 [CrossRef]
    [Google Scholar]
  14. Könneke M., Bernhard A. E., de la Torre J. R., Walker C. B., Waterbury J. B., Stahl D. A. 2005; Isolation of an autotrophic ammonia-oxidizing marine archaeon. Nature 437:543–546 [CrossRef]
    [Google Scholar]
  15. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  16. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S., Jobb G. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  17. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  18. Näther D. J. 2003; Elektronenmikroskopische und biochemische Untersuchungen an der äußeren Membran von Ignicoccus sp . Diploma thesis Institute for Microbiology, University of Regensburg, Germany (in German;
  19. Näther D. J., Rachel R. 2004; The outer membrane of the hyperthermophilic archaeon Ignicoccus : dynamics, ultrastructure and composition. Biochem Soc Trans 32:199–203 [CrossRef]
    [Google Scholar]
  20. Pley U., Schipka J., Gambacorta A., Jannasch H. W., Fricke H., Rachel R., Stetter K. O. 1991; Pyrodictium abyssi sp. nov. represents a novel heterotrophic marine archaeal hyperthermophile growing at 110 °C. Syst Appl Microbiol 14:245–253 [CrossRef]
    [Google Scholar]
  21. Rachel R., Wyschkony I., Riehl S., Huber H. 2002; The ultrastructure of Ignicoccus : evidence for a novel outer membrane and for intracellular vesicle budding in an archaeon. Archaea 1:9–18 [CrossRef]
    [Google Scholar]
  22. Rieger G., Müller K., Hermann R., Stetter K. O., Rachel R. 1997; Cultivation of hyperthermophilic archaea in cellulose capillaries resulting in improved preservation of fine structure. Arch Microbiol 168:373–379 [CrossRef]
    [Google Scholar]
  23. Schägger H., von Jagow C. 1987; Tricine-SDS-PAGE for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:223–231
    [Google Scholar]
  24. Stetter K. O. 1989; Extremely thermophilic chemolithoautotrophic archaebacteria. In Autotrophic Bacteria pp  167–176 Edited by Schlegel H. G., Bowien B. Berlin: Springer;
    [Google Scholar]
  25. Stetter K. O. 1992; Life at the upper temperature border. In Frontiers of Life pp  195–219 Edited by Trân Than Vân J., Trâ Than Vân K., Mounolou J. C., Schneider J., McKay C. Gif-sur-Yvette: Editions Frontières;
    [Google Scholar]
  26. Stetter K. O., König H., Stackebrandt E. 1983; Pyrodictium gen. nov., a new genus of submarine disc-shaped sulphur reducing archaebacteria growing optimally at 105 °C. Syst Appl Microbiol 4:535–551 [CrossRef]
    [Google Scholar]
  27. Völkl P., Huber R., Drobner E., Rachel R., Burggraf S., Trincone A., Stetter K. O. 1993; Pyrobaculum aerophilum sp. nov., a novel nitrate-reducing hyperthermophilic archaeum. Appl Environ Microbiol 59:2918–2926
    [Google Scholar]
  28. Waters E., Hohn M. J., Ahel I., Graham D. E., Adams M. D., Barnstead M., Beeson K. Y., Bibbs L., Bolanos R. other authors 2003; The genome of Nanoarchaeum equitans : insights into early archaeal evolution and derived parasitism. Proc Natl Acad Sci U S A 100:12984–12988 [CrossRef]
    [Google Scholar]
  29. Wildgruber G., Thomm M., König H., Ober K., Ricchiuto T., Stetter K. O. 1982; Methanoplanus limicola , a plate-shaped methanogen representing a novel family, the Methanoplanaceae . Arch Microbiol 132:31–36 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64721-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64721-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed