1887

Abstract

A mesophilic, strictly anaerobic bacterium, strain Sjm18-20, was isolated from the alimentary canal of a Japanese corbicula clam. Cells of strain Sjm18-20 were Gram-negative, non-sporulating, straight to slightly curved rods, 2.5–6.0 μm long, and were motile with oscillatory movements by means of peritrichous flagella. Cells elongated to 30 μm after prolonged cultivation. Optimum growth was observed at 30 °C and pH 6.0–6.5. Growth occurred below 4.0 % (w/v) NaCl. Strain Sjm18-20 produced acid from -glucose and a few pentoses such as -arabinose, -ribose and -xylose. -Valeric acid was the major end product from glucose. The genomic DNA G+C content of strain Sjm18-20 was 52.9 mol%. Phylogenetic analysis based on the 16S rRNA gene revealed that strain Sjm18-20 could be accommodated in clostridial cluster IV of the low-G+C-content Gram-positive bacteria and that the closest neighbour of this organism (92.6–92.9 % similarity) was the cloned 16S rRNA gene sequence of a not-yet cultured bacterium, thought to represent . The nearest cultivated neighbours of strain Sjm18-20 were DSM 6740 and T2-7, with sequence similarities of 91.3 and 89.1 %, respectively. On the basis of phenotypic features and phylogenetic position, it is proposed that this isolate represents a novel species in a new genus, gen. nov., sp. nov. The type strain of is Sjm18-20 (=NBRC 101213 =DSM 18026).

Keyword(s): DMA, dimethylacetal
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2007-08-01
2019-10-19
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