1887

Abstract

A Gram-positive, rod-shaped, non-spore-forming and strictly aerobic bacterium (Gsoil 161) was isolated from soil of a ginseng field in Pocheon Province, South Korea. The novel isolate was characterized using a polyphasic approach in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 161 was shown to belong to the family and was related to (98.0 % similarity to the type strain), (97.6 %), (97.0 %) and (96.7 %); the sequence similarity with other species within the family was less than 94.4 %. It was characterized chemotaxonomically as having -2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-9(H) as the predominant menaquinone and C, 10-methyl C (tuberculostearic acid), C 2-OH, 10-methyl C and 10-methyl-C as the major fatty acids. The G+C content of the genomic DNA was 65.5 mol%. These chemotaxonomic properties and phenotypic characteristics support the affiliation of strain Gsoil 161 to the genus . Results of physiological and biochemical tests enabled strain Gsoil 161 to be differentiated genotypically and phenotypically from currently known species. Therefore, strain Gsoil 161 represents a novel species, for which the name sp. nov. is proposed. The type strain is strain Gsoil 161 (=KCTC 19131=DSM 17939=CCUG 52476).

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2007-04-01
2019-10-23
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