1887

Abstract

Two bacterial strains isolated from different hospitals in Japan were subjected to a polyphasic analysis. Strains IFM 0406 and IFM 0706, producers of novel terpenoid antibiotics, were found to have morphological, biochemical, physiological and chemotaxonomic properties consistent with their classification in the genus , except for the presence of MK-8(H) as one of the predominant menaquinones in addition to the major menaquinone MK-8(H). The 16S rRNA gene sequence similarity of strains IFM 0406 and IFM 0706 was 99.9 %, and the closest members of to these strains were the type strains of and , showing similarity of 97.5 and 97.1 %, respectively. Based on their characteristic phenotypic and phylogenetic properties, a novel species of the genus , sp. nov. is proposed for the two strains. The type strain is IFM 0706 (=JCM 13033=DSM 44935=NBRC 100888).

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2007-07-01
2021-02-28
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References

  1. Chapin K. C., Murray P. R. 1999; Stains. In Manual of Clinical Microbiology pp  1674–1686 Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  2. Chun J., Goodfellow M. 1995; A phylogenetic analysis of genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Goodfellow M. 1998; Nocardia and related genera. In Topley and Wilson's Microbiology and Microbial Infections 9th edn., vol. 2, Systematic Bacteriology pp  463–489 Edited by Balows A., Duerden B. I. London: Arnold;
    [Google Scholar]
  5. Goodfellow M., Pirouz T. 1982; Numerical classification of sporoactinomycetes containing meso -diaminopimelic acid in the cell wall. J Gen Microbiol 128:503–527
    [Google Scholar]
  6. Goodfellow M., Isik K., Yates E. 1999; Actinomycete systematics: an unfinished synthesis. Nova Acta Leopold 80:47–82
    [Google Scholar]
  7. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  8. Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. 2004; The complete genomic sequence of Nocardia farcinica IFM 10152. Proc Natl Acad Sci U S A 101:14925–14930 [CrossRef]
    [Google Scholar]
  9. Kageyama A., Poonwan N., Yazawa K., Mikami Y., Nishimura K. 2004a; Nocardia asiatica sp. nov., isolated from patients with nocardiosis in Japan and clinical specimens from Thailand. Int J Syst Evol Microbiol 54:125–130 [CrossRef]
    [Google Scholar]
  10. Kageyama A., Yazawa K., Nishimura N., Mikami Y. 2004b; Nocardia inohanensis sp. nov., Nocardia yamanashiensis sp. nov. and Nocardia niigatensis sp. nov., isolated from clinical specimens. Int J Syst Evol Microbiol 54:563–569 [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  12. Kimura M., Ohta T. 1972; On the stochastic model for estimation of mutational distance between homologous proteins. J Mol Evol 2:87–90 [CrossRef]
    [Google Scholar]
  13. Kinoshita N., Homma Y., Igarashi M., Ikeno S., Hori M., Hamada M. 2000; Nocardia vinacea sp. nov.. Actinomycetologica 151–5
    [Google Scholar]
  14. Klatte S., Rainey F. A., Kroppenstedt R. M. 1994; Transfer of Rhodococcus aichiensis Tsukamura 1982 and Nocardia amarae Lechevalier and Lechevalier 1974 to the genus Gordona as Gordona aichiensis comb nov. and Gordona amarae comb nov. Int J Syst Bacteriol 44:769–773 [CrossRef]
    [Google Scholar]
  15. Komatsu K., Tsuda M., Tanaka Y., Mikami Y., Kobayashi J. 2004; Absolute stereochemistry of immunosuppressive macrolide brasilinolide A and its congener brasilinolide C. J Org Chem 69:1535–1541 [CrossRef]
    [Google Scholar]
  16. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy pp  227–291 Edited by Dietz A., Thayer D. W. Fairfax, VA: Society for Industrial Microbiology;
    [Google Scholar]
  17. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233 [CrossRef]
    [Google Scholar]
  18. Miyadoh M. 2001; Identification procedure at the genus level. In Identification Manual of Actinomycetes pp  9–19 Edited by Miyadoh S., Hamada M., Hotta K., Kudo T., Seino A., Suzuki K., Yokota A. Tokyo: Business Center for Academic Societies Japan;
    [Google Scholar]
  19. Poonwan N., Mekha N., Yazawa K., Thunyaharn S., Yamanaka A., Mikami Y. 2005; Characterization of clinical isolates of pathogenic Nocardia strains and related actinomycetes in Thailand from 1996 to 2003. Mycopathologia 159:361–368 [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:1–6
    [Google Scholar]
  22. Seo J. P., Lee S. D. 2006; Nocardia harenae sp. nov., an actinomycete isolated from beach sand. Int J Syst Evol Microbiol 56:2203–2207 [CrossRef]
    [Google Scholar]
  23. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  24. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  25. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  26. Tanaka Y., Komaki H., Yazawa K., Mikami Y., Nemoto A., Kadowaki K., Shigemori H., Kobayashi J. 1997; A new macrolide antibiotic produced by Nocardia brasiliensis : producing strain, isolation and biological activity. J Antibiot 50:1036–1041 [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
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