1887

Abstract

Moderately thermophilic, acidophilic, spore-forming bacteria (146 strains) were isolated from various beverages and environments. Based on the results of sequence analysis of the hypervariable region of the 16S rRNA gene, eight of the strains represent novel species of the genus . These strains were designated 3-A191, 4-A336, 5-A83J, 5-A167N, 5-A239-2O-A, E-8, RB718 and S-TAB. Phylogenetic analyses of 16S rRNA and DNA gyrase B subunit () nucleotide sequences confirmed that the eight strains belonged to the clade. Cells of the eight strains were Gram-positive or Gram-variable, strictly aerobic and rod-shaped. The strains grew well under acidic and moderately thermal conditions, produced acid from various sugars, contained menaquinone 7 as the major isoprenoid quinone and did not produce guaiacol. -Alicyclic fatty acids were the predominant lipid component of strains 4-A336, 5-A83J, 5-A167N, RB718 and S-TAB. No -alicyclic fatty acids were detected in strains 3-A191, 5-A239-2O-A or E-8, but iso- and anteiso-branched fatty acids and small amounts of straight-chain saturated fatty acids were detected instead. According to the DNA–DNA hybridization data and distinct morphological, physiological, chemotaxonomical and genetic traits, the eight strains represent six novel species within the genus , for which the following names are proposed: sp. nov. (type strain 3-A191=DSM 17975=IAM 15224), sp. nov. (type strain S-TAB=DSM 17978=IAM 15229), sp. nov. (type strain 5-A83J=DSM 17979=IAM 15227), sp. nov. (type strain 5-A239-2O-A=DSM 17980=IAM 15370), sp. nov. (type strain RB718=DSM 17974=IAM 15230) and sp. nov. (type strain 4-A336=DSM 17981=IAM 15226).

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2007-06-01
2020-01-23
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vol. , part 6, pp. 1276 - 1285

DNA base compositions and DNA-DNA hybridization values between the novel isolates and type strains of related species. [PDF](18 KB)



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