1887

Abstract

A novel Gram-positive bacterium, strain 40, was isolated in the course of identifying bacteria from infective juveniles of the entomopathogenic nematode . Based on 16S rRNA gene analysis, strain 40 was found to be related to the type strains of recognized species of the genus , family . The 16S rRNA gene sequence similarity values of strain 40 and IAM 14851, LMG 23118, LMG 23117, DSM 8803, CIP 108389 and CIP 108388, respectively, were 97.3, 97.5, 97.6, 97.6, 97.6 and 98.5 %. Chemotaxonomic analysis also supported the affiliation of strain 40 to the genus : the major menaquinone was MK-11, the peptidoglycan cross-linkage was of the B-type, the cell wall diamino acid was -diaminobutyric acid and the major fatty acids were anteiso-C (42 %), anteiso-C (34 %) and iso-C (16 %). Based upon the biochemical and genomic analyses, strain 40 is sufficiently distinct from the type strains of recognized species to warrant the description of a novel species, for which the name sp. nov. is proposed. The type strain is strain 40 (=DSM 17402=CIP 108831).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64683-0
2007-04-01
2020-09-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/4/682.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64683-0&mimeType=html&fmt=ahah

References

  1. Akhurst R. J. 1980; Morphological and functional dimorphism in Xenorhabdus spp., bacteria symbiotically associated with the insect pathogenic nematodes Neoaplectana and Heterorhabditis . J Gen Microbiol 121:303–309
    [Google Scholar]
  2. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5.1. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  3. Ganguly S., Singh L. K. 2000; Steinernema thermophilum sp. n. (Rhabditida Steinernematidae) from India. Int J Nematol 10:183–191
    [Google Scholar]
  4. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov. a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  5. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  6. Lin Y.-C., Uemori K., de Briel D. A., Arunpairojana V., Yokota A. 2004 Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae . Int J Syst Evol Microbiol 54, 1669–1676 [CrossRef]
  7. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McCaughey M. J., Woese C. R. 1997; The RDP (Ribosomal Database Project). Nucleic Acids Res 25:109–111 [CrossRef]
    [Google Scholar]
  8. MIDI 1999 Sherlock Microbial Identification System Operating Manual , version 3.0 Newark, DE: MIDI;
    [Google Scholar]
  9. Morais P. V., Francisco R., Branco R., Chung A. P., da Costa M. S. 2004; Leucobacter chromiireducens sp. nov., and Leucobacter aridicollis sp. nov., two new species isolated from a chromium contaminated environment. Syst Appl Microbiol 27:646–652 [CrossRef]
    [Google Scholar]
  10. Morais P. V., Paulo C., Francisco R., Branco R., Chung A. P., da Costa M. S. 2006; Leucobacter luti sp. nov., and Leucobacter alluvii sp. nov., two new species of the genus Leucobacter isolated under chromium stress. Syst Appl Microbiol 29:414–421 [CrossRef]
    [Google Scholar]
  11. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and distinct actinomycete lineage; proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  13. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  14. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  15. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  16. Somvanshi V. S., Lang E., Sträubler B., Spröer C., Schumann P., Ganguly S., Saxena A. K., Stackebrandt E. 2006; Providencia vermicola sp. nov., isolated from infective juveniles of the entomopathogenic nematode Steinernema thermophilum . Int J Syst Evol Microbiol 56:629–633 [CrossRef]
    [Google Scholar]
  17. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiology Today 33:152–155
    [Google Scholar]
  18. Takeuchi M., Weiss N., Schumann P., Yokota A. 1996 Leucobacter komagatae gen. nov., sp. nov., a new aerobic Gram-positive, nonsporulating rod with 2,4-diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46967–971 [CrossRef]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64683-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64683-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error