RT Journal Article SR Electronic(1) A1 Han, Xiang Y. A1 Han, Faye S. A1 Segal, JonathanYR 2008 T1 Chromobacterium haemolyticum sp. nov., a strongly haemolytic species JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 6 SP 1398 OP 1403 DO https://doi.org/10.1099/ijs.0.64681-0 PB Microbiology Society, SN 1466-5034, AB A Gram-negative bacterium, strain MDA0585T, isolated from a sputum culture, was characterized by a polyphasic approach. The 16S rRNA gene and a conserved portion of the DNA gyrase A gene were sequenced and analysed phylogenetically. Strain MDA0585T showed the closest relationships with Chromobacterium violaceum ATCC 12472T and Chromobacterium subtsugae PRAA4-1T (96.1 % and 96.3 % 16S rRNA gene sequence similarity, respectively). The cellular fatty acids of strain MDA0585T consisted mainly of C16 : 0, C16 : 1 ω7c and C16 : 1 ω6c (summed feature 3) and C18 : 1 ω7c and C18 : 1 ω6c (summed feature 8), a profile that was similar to, but distinguishable from, those of C. violaceum ATCC 12472T and C. subtsugae PRAA4-1T. In culture, strain MDA0585T differed from C. violaceum and C. subtsugae in several ways: lack of violet pigmentation, the ability to haemolyse sheep blood, differences in several biochemical reactions and higher resistance to antibiotics. The culture supernatant of strain MDA0585T also caused remarkable haemolysis of human erythrocytes. These results suggest that strain MDA0585T represents a novel species within the genus Chromobacterium, for which the name Chromobacterium haemolyticum sp. nov. is proposed. The type strain is MDA0585T (=CCUG 53230T=JCM 14163T=DSM 19808T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64681-0