1887

Abstract

A Gram-negative bacterium, strain MDA0585, isolated from a sputum culture, was characterized by a polyphasic approach. The 16S rRNA gene and a conserved portion of the DNA gyrase A gene were sequenced and analysed phylogenetically. Strain MDA0585 showed the closest relationships with ATCC 12472 and PRAA4-1 (96.1 % and 96.3 % 16S rRNA gene sequence similarity, respectively). The cellular fatty acids of strain MDA0585 consisted mainly of C, C 7 and C 6 (summed feature 3) and C 7 and C 6 (summed feature 8), a profile that was similar to, but distinguishable from, those of ATCC 12472 and PRAA4-1. In culture, strain MDA0585 differed from and in several ways: lack of violet pigmentation, the ability to haemolyse sheep blood, differences in several biochemical reactions and higher resistance to antibiotics. The culture supernatant of strain MDA0585 also caused remarkable haemolysis of human erythrocytes. These results suggest that strain MDA0585 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is MDA0585 (=CCUG 53230=JCM 14163=DSM 19808).

Keyword(s): RBC, red blood cells
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2008-06-01
2019-10-18
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Cellular fatty acid contents of strain MDA0585 and ATCC 12472 . [ PDF] 55 KB

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Graphs showing the time course of the growth of strain MDA0585 and the lysis of human red blood cells by the culture supernatants. [ PDF] 20 KB

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