1887

Abstract

A thermotolerant acetic acid bacterium, designated strain CWBI-B418, isolated in Senegal from mango fruit (), was characterized in detail by means of genotypic and phenotypic methods. The novel strain was strictly aerobic and exhibited optimal growth on YGM medium at 35 °C. Cells were Gram-negative, motile and coccoid. The strain was assigned to the genus on the basis of 16S rRNA gene sequence analysis. DNA–DNA hybridization experiments with its phylogenetically closest relatives showed that strain CWBI-B418 represented a novel genospecies. The DNA G+C content of strain CWBI-B418 was 56.0 mol%. Phenotypic characteristics enabling the differentiation of strain CWBI-B418 from phylogenetically related species were: production of 2-keto--gluconic acid from -glucose, but not 5-keto--gluconic acid, production of catalase but not oxidase, growth on yeast extract with 30 % -glucose, growth with ammonium as sole nitrogen source with ethanol as carbon source, utilization of glycerol and ethanol but not maltose or methanol as carbon sources, and growth in the presence of 10 % ethanol. Based on the genotypic and phenotypic data presented, strain CWBI-B418 clearly represents a novel species, for which the name sp. nov. is proposed. The type strain is CWBI-B418 (=LMG 23690=DSM 18889).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64678-0
2007-07-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/7/1576.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64678-0&mimeType=html&fmt=ahah

References

  1. Cleenwerck, I., Vandemeulebroecke, K., Janssens, D. & Swings, J. ( 2002; ). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov. Int J Syst Evol Microbiol 52, 1551–1558.[CrossRef]
    [Google Scholar]
  2. De Ley, J., Gillis, M. & Swings, J. ( 1984; ). Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 268–278. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  3. Dutta, D. & Gachhui, R. ( 2006; ). Novel nitrogen-fixing Acetobacter nitrogenifigens sp. nov., isolated from Kombucha tea. Int J Syst Evol Microbiol 56, 1899–1903.[CrossRef]
    [Google Scholar]
  4. Ezaki, T., Yashimoto, T. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K. ( 1998; ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef]
    [Google Scholar]
  7. Hucker, G. J. & Conn, H. J. ( 1923; ). Methods of Gram staining. NYS Agric Exp Sta Tech Bull 93, 3–37.
    [Google Scholar]
  8. Jojima, Y., Mihara, Y., Suzuki, S., Yokozeki, K., Yamanaka, S. & Fudou, R. ( 2004; ). Saccharibacter floricola gen. nov., sp. nov., a novel osmophilic acetic acid bacterium isolated from pollen. Int J Syst Evol Microbiol 54, 2263–2267.[CrossRef]
    [Google Scholar]
  9. Lisdiyanti, P., Kawasaki, H., Seki, T., Yamada, Y., Uchimura, T. & Komagata, K. ( 2000; ). Systematic study of the genus Acetobacter with descriptions of Acetobacter indonensiensis sp. nov., Acetobacter tropicalis sp. nov., Acetobacter orleanensis (Henneberg 1906) comb. nov., Acetobacter lovaniensis (Frateur 1950) comb. nov., and Acetobacter estunensis (Carr 1958) comb. nov. J Gen Appl Microbiol 46, 147–165.[CrossRef]
    [Google Scholar]
  10. Lisdiyanti, P., Kawasaki, H., Seki, T., Yamada, Y., Uchimura, T. & Komagata, K. ( 2001; ). Identification of Acetobacter strains isolated from Indonesian sources, and proposals of Acetobacter syzygii sp. nov., Acetobacter cibinongensis sp. nov., and Acetobacter orientalis sp. nov. J Gen Appl Microbiol 47, 119–131.[CrossRef]
    [Google Scholar]
  11. Loganathan, P. & Nair, S. ( 2004; ). Swaminathania salitorans gen. nov., sp. nov., a salt-tolerant, nitrogen-fixing and phosphate-solubilizing bacterium from wild rice (Porterisia coarctata Tateoka). Int J Syst Evol Microbiol 54, 1185–1190.[CrossRef]
    [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. Ndoye, B., Lebecque, S., Dubois-Dauphin, R., Tounkara, L., Guiro, T.-A., Kere, C., Diawara, B. & Thonart, P. ( 2006; ). Thermoresistant properties of acetic acid bacteria isolated from tropical products of Sub-Saharan Africa and destined to industrial vinegar. Enzyme Microb Technol 39, 916–923.[CrossRef]
    [Google Scholar]
  14. Ndoye, B., Weekers, F., Diawara, B., Guiro, A. T. & Thonart, P. ( 2007; ). Survival and preservation after freeze-drying process of thermoresistant acetic acid bacteria (TAAB) isolated from tropical products of Subsaharan Africa. J Food Eng 79, 1374–1382.[CrossRef]
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Silva, L. R., Cleenwerck, I., Rivas, R., Swings, J., Trujillo, M. E., Willems, A. & Velázquez, E. ( 2006; ). Acetobacter oeni sp. nov., isolated from spoiled red wine. Int J Syst Evol Microbiol 56, 21–24.[CrossRef]
    [Google Scholar]
  17. Sokollek, S. J., Hertel, C. & Hammes, W. P. ( 1998; ). Description of Acetobacter oboediens sp. nov. and Acetobacter pomorum sp. nov., two new species isolated from industrial vinegar fermentations. Int J Syst Bacteriol 48, 935–940.[CrossRef]
    [Google Scholar]
  18. Sow, N. M., Dubois-Dauphin, R., Roblain, D., Guiro, A. T. & Thonart, Ph. ( 2005; ). Polyphasic identification of a new thermotolerant species of lactic acid bacteria isolated from chicken faeces. Afr J Biotechnol 4, 409–421.
    [Google Scholar]
  19. Stackebrandt, E., Frederiksen, W., Garrity, G. M., Grimont, P. A. D., Kämpfer, P., Maiden, M. C. J., Nesme, X., Rosselló-Mora, R., Swings, J. & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  20. Trček, J. & Teuber, M. ( 2002; ). Genetic and restriction analysis of the 16S–23S rDNA internal transcribed spacer regions of the acetic acid bacteria. FEMS Microbiol Lett 208, 69–75.
    [Google Scholar]
  21. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  22. Wilson, K. ( 1987; ). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1.–2.4.5. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Green Publishing & Wiley-Interscience.
  23. Yukphan, P., Malimas, T., Potacharoen, W., Tanasupawat, S., Tanticharoen, M. & Yamada, Y. ( 2005; ). Neoasaia chiangmaiensis gen. nov., sp. nov., a novel osmotolerant acetic acid bacterium in the α-Proteobacteria. J Gen Appl Microbiol 51, 301–311.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64678-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64678-0
Loading

Data & Media loading...

Supplements

vol. , part 7, pp. 1576 - 1581

Oxidation of carbon sources by strain CWBI-B418 and . [ PDF] (18 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error