1887

Abstract

A Gram-negative, extremely halophilic, coccoid archaeal strain, CM5, was isolated from a crude sea-salt sample collected near Qingdao, China. The organism grew optimally at 35–40 °C and pH 6.0 in the presence of 20 % (w/v) NaCl. Its colonies were red in colour and it could use glucose as a sole carbon source for growth. The 16S rRNA gene sequence of CM5 was most closely related to those of species. Its pattern of antibiotic susceptibility was similar to those of other described species. Biochemical tests revealed no sign of HS production or gelatin liquefaction. The main polar lipids of strain CM5 were phosphatidylglycerol, phosphatidylglycerol methylphosphate and sulfated diglycosyl diether. No phosphatidylglycerol sulfate was present. The DNA G+C content of strain CM5 was 61.2 mol% and it gave DNA–DNA reassociation values of 33.7, 57.1 and 29.6 %, respectively, with DSM 8989, DSM 14522 and ATCC 17082. Based on its morphological and chemotaxonomic properties and phylogenetic analysis of 16S rRNA gene sequence data, we propose that CM5 should be classified within a novel species, sp. nov., with strain CM5 (=CGMCC 1.4243=JCM 13587) as the type strain.

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2007-03-01
2019-10-17
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