%0 Journal Article %A Wang, Qian-fu %A Li, Wei %A Liu, Yan-li %A Cao, Hai-hua %A Li, Zhao %A Guo, Guang-qin %T Bacillus qingdaonensis sp. nov., a moderately haloalkaliphilic bacterium isolated from a crude sea-salt sample collected near Qingdao in eastern China %D 2007 %J International Journal of Systematic and Evolutionary Microbiology, %V 57 %N 5 %P 1143-1147 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.64668-0 %I Microbiology Society, %X A moderately haloalkaliphilic, Gram-positive bacterium, designated as strain CM1T, was isolated from a crude sea-salt sample collected near Qingdao in eastern China. Strain CM1T was found to grow optimally at 37 °C and pH 9.0. It was shown to be aerobic, rod-shaped and capable of growth at salinities of 2.5–20 % (w/v) NaCl (optimum, 12 %). The genomic DNA G+C content was about 48 mol%. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 and the major isoprenoid quinones were MK-7(H2) and MK-6(H2). Phylogenetic analyses based on 16S rRNA gene sequences revealed that CM1T is a member of the genus Bacillus and has less than 95.2 % gene sequence similarity to the most closely related strain, Bacillus salarius BH169T. Its DNA–DNA reassociation value with respect to B. salarius BH169T was 35.4 %. On the basis of phenotypic and molecular properties, strain CM1T represents a novel Bacillus species, for which the name Bacillus qingdaonensis sp. nov. is proposed. The type strain is CM1T (=CGMCC 1.6134T=JCM 14087T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64668-0