1887

Abstract

A moderately haloalkaliphilic, Gram-positive bacterium, designated as strain CM1, was isolated from a crude sea-salt sample collected near Qingdao in eastern China. Strain CM1 was found to grow optimally at 37 °C and pH 9.0. It was shown to be aerobic, rod-shaped and capable of growth at salinities of 2.5–20 % (w/v) NaCl (optimum, 12 %). The genomic DNA G+C content was about 48 mol%. The major cellular fatty acids were anteiso-C, anteiso-C and iso-C and the major isoprenoid quinones were MK-7(H) and MK-6(H). Phylogenetic analyses based on 16S rRNA gene sequences revealed that CM1 is a member of the genus and has less than 95.2 % gene sequence similarity to the most closely related strain, BH169. Its DNA–DNA reassociation value with respect to BH169 was 35.4 %. On the basis of phenotypic and molecular properties, strain CM1 represents a novel species, for which the name sp. nov. is proposed. The type strain is CM1 (=CGMCC 1.6134=JCM 14087).

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2007-05-01
2019-10-23
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vol. , part 5, pp. 1143 - 1147

Phylogenetic trees based on maximum-parsimony and minimum-evolution analyses of the 16S rRNA gene sequences of CM1 and related species. [PDF](34 KB)



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