1887

Abstract

Two Gram-negative, milky-white-pigmented, motile, slightly curved rod-shaped bacterial isolates, UMS-37 and UMS-40, were isolated from rhizosphere soil of wild edible greens cultivated on Ulleung island, Korea, and their taxonomic positions were investigated by a polyphasic approach. They grew optimally at 25–30 °C and contained Q-8 as the predominant ubiquinone. The major cellular fatty acids (>10 % of total fatty acids) were C, cyclo C and C 7 and/oriso-C 2-OH. The DNA G+C contents of the two isolates were 59.8 and 60.0 mol%. Isolates UMS-37 and UMS-40 exhibited no difference in their 16S rRNA gene sequences and possessed a mean DNA–DNA relatedness level of 94 %; they exhibited 16S rRNA gene sequence similarity levels of 96.8–98.2 % to the type strains of recognized species. Phylogenetic analyses based on 16S rRNA gene sequences showed that isolates UMS-37 and UMS-40 formed a distinct phylogenetic lineage within the genus . DNA–DNA relatedness levels between isolates UMS-37 and UMS-40 and the type strains of some phylogenetically related species were in the range 3–56 %. On the basis of differences in phenotypic properties and phylogenetic distinctiveness and genomic data, isolates UMS-37 and UMS-40 were classified in the genus within a novel species, for which the name sp. nov. is proposed, with the type strain UMS-37 (=KCTC 12558 =CIP 108917).

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2007-10-01
2021-03-03
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References

  1. Aragno M., Schlegel H. G. 1978; Aquaspirillum autotrophicum , a new species of hydrogen-oxidizing, facultatively autotrophic bacteria. Int J Syst Bacteriol 28:112–116 [CrossRef]
    [Google Scholar]
  2. Baldani J. I., Baldani V. L. D., Seldin L., Döbereiner J. 1986; Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium. Int J Syst Bacteriol 36:86–93 [CrossRef]
    [Google Scholar]
  3. Baldani J. I., Pot B., Kirchhof G., Falsen E., Baldani V. L. D., Olivares F. L., Hoste B., Kersters K., Hartmann A. other authors 1996; Emended description of Herbaspirillum ; inclusion of[ Pseudomonas ] rubrisubalbicans ,a mild plant pathogen, as Herbaspirillum rubrisubalbicans comb. nov.; and classification of a group of clinical isolates (EF group 1) as Herbaspirillum species 3. Int J Syst Bacteriol 46:802–810 [CrossRef]
    [Google Scholar]
  4. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  5. Ding L., Yokota A. 2004 Proposals of Curvibacter gracilis gen. nov., sp. nov. and Herbaspirillum putei sp. nov. for bacterial isolates isolated from well water and reclassification of [ Pseudomonas ] huttiensis , [ Pseudomonas ] lanceolata , [ Aquaspirillum ] delicatum and [ Aquaspirillum ] autotrophicum as Herbaspirillum huttiense comb. nov., Curvibacter lanceolatus comb. nov., Curvibacter delicatus comb. nov. and Herbaspirillum autotrophicum comb. nov. Int J Syst Evol Microbiol 54, 2223–2230 [CrossRef]
  6. Döbereiner J. 1995; Isolation and identification of aerobic nitrogen-fixing bacteria from soil and plants. In Methods in Applied Soil Microbiology and Biochemistry pp 134–141 Edited by Alef K., Nannipieri P. London: Academic Press;
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial isolates. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  8. Im W.-T., Bae H.-S., Yokota A., Lee S. T. 2004; Herbaspirillum chlorophenolicum sp. nov., a 4-chlorophenol-degrading bacterium. Int J Syst Evol Microbiol 54:851–855 [CrossRef]
    [Google Scholar]
  9. Kirchhof G., Eckert B., Stoffels M., Baldani J. I., Reis V. M., Hartmann A. 2001; Herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in C4-fibre plants. Int J Syst Evol Microbiol 51:157–168
    [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  11. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  12. Leifson E. 1962; The bacterial flora of distilled and stored water. III. New species of the genera Corynebacterium , Flavobacterium , Spirillum and Pseudomonas . Int Bull Bacteriol Nomencl Taxon 12:161–170
    [Google Scholar]
  13. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  14. Poly F., Monrozier L. J., Bally R. 2001; Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil. Res Microbiol 152:95–103 [CrossRef]
    [Google Scholar]
  15. Rothballer M., Schmid M., Klein I., Gattinger A., Grundmann S., Hartmann A. 2006; Herbaspirillum hiltneri sp. nov., isolated from surface-sterilized wheat roots. Int J Syst Evol Microbiol 56:1341–1348 [CrossRef]
    [Google Scholar]
  16. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  17. Stoltzfus J. R., So R., Malarvithi P. P., Ladha J. K., de Bruijn F. J. 1997; Isolation of endophytic bacteria from rice and assessment of their potential for supplying rice with biologically fixed nitrogen. Plant Soil 194:25–36 [CrossRef]
    [Google Scholar]
  18. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  19. Valverde A., Velázquez E., Gutiérrez C., Cervantes E., Ventosa A., Igual J.-M. 2003; Herbaspirillum lusitanum sp. nov., a novel nitrogen-fixing bacterium associated with root nodules of Phaseolus vulgaris . Int J Syst Evol Microbiol 53:1979–1983 [CrossRef]
    [Google Scholar]
  20. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  21. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora isolates by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  22. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  23. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef]
    [Google Scholar]
  24. Yurkov V., Stackebrandt E., Holmes A., Fuerst J. A., Hugenholtz P., Golecki J., Gad'on N., Gorlenko V. M., Kompantseva E. I., Drews G. 1994; Phylogenetic positions of novel aerobic, bacteriochlorophyll a -containing bacteria and description of Roseococcus thiosulfatophilus gen.nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov.. Int J Syst Bacteriol 44427–434 [CrossRef]
    [Google Scholar]
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