1887

Abstract

Two Gram-negative, milky-white-pigmented, motile, slightly curved rod-shaped bacterial isolates, UMS-37 and UMS-40, were isolated from rhizosphere soil of wild edible greens cultivated on Ulleung island, Korea, and their taxonomic positions were investigated by a polyphasic approach. They grew optimally at 25–30 °C and contained Q-8 as the predominant ubiquinone. The major cellular fatty acids (>10 % of total fatty acids) were C, cyclo C and C 7 and/oriso-C 2-OH. The DNA G+C contents of the two isolates were 59.8 and 60.0 mol%. Isolates UMS-37 and UMS-40 exhibited no difference in their 16S rRNA gene sequences and possessed a mean DNA–DNA relatedness level of 94 %; they exhibited 16S rRNA gene sequence similarity levels of 96.8–98.2 % to the type strains of recognized species. Phylogenetic analyses based on 16S rRNA gene sequences showed that isolates UMS-37 and UMS-40 formed a distinct phylogenetic lineage within the genus . DNA–DNA relatedness levels between isolates UMS-37 and UMS-40 and the type strains of some phylogenetically related species were in the range 3–56 %. On the basis of differences in phenotypic properties and phylogenetic distinctiveness and genomic data, isolates UMS-37 and UMS-40 were classified in the genus within a novel species, for which the name sp. nov. is proposed, with the type strain UMS-37 (=KCTC 12558 =CIP 108917).

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2007-10-01
2020-01-29
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