1887

Abstract

Several strains of the fungus harbour endosymbiotic bacteria for the production of the causal agent of rice seedling blight, rhizoxin, and the toxic cyclopeptide rhizonin. and isolated endobacteria were selected for freeze–fracture electron microscopy, which allowed visualization of bacterial cells within the fungal cytosol by their two parallel-running envelope membranes and by the fine structure of the lipopolysaccharide layer of the outer membrane. Two representatives of bacterial endosymbionts were chosen for phylogenetic analyses on the basis of full 16S rRNA gene sequences, which revealed that the novel fungal endosymbionts formed a monophyletic group within the genus . Inter-sequence similarities ranged from 98.94 to 100 %, and sequence similarities to members of the group, the closest neighbours, were 96.74–97.38 %. In addition, the bacterial strains were distinguished from their phylogenetic neighbours by their fatty acid profiles and other biochemical characteristics. The phylogenetic studies based on 16S rRNA gene sequence data, together with conclusive DNA–DNA reassociation experiments, strongly support the proposal that these strains represent two novel species within the genus , for which the names sp. nov. (type strain, HKI 454=DSM 19002=CIP 109453) and sp. nov. (type strain, HKI 456=DSM 19003=CIP 109454) are proposed.

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2007-11-01
2020-01-22
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