1887

Abstract

A novel marine bacterial strain, HY1, was isolated from sediment of the South China Sea. The strain was aerobic and heterotrophic and formed saffron yellow-pigmented colonies on marine agar 2216. Cells were non-motile, Gram-negative rods, frequently occurring in chains. searches revealed that the 16S rRNA gene sequence of strain HY1 showed high similarity with those of members of the genera (91.7–93.8 %) and (92.6–93.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain clustered with members of both and and phylogenetic trees constructed using three different methods (neighbour-joining, maximum-parsimony and minimum-evolution) indicated that strain HY1 clustered more frequently with members of the genus . The DNA G+C content of strain HY1 was 44.4 mol% and its major cellular fatty acids (⩾5 % of the total fatty acids) were iso-15 : 1 (5.0 %), iso-15 : 0 (6.8 %), anteiso-15 : 0 (6.4 %), 15 : 0 (10.4 %), iso-16 : 0 (13.5 %), summed feature 3 (comprising iso-15 : 0 2-OH and/or 16 : 17; 6.3 %), iso-17 : 0 3-OH (5.2 %) and 17 : 0 2-OH (5.0 %). Cells contained menaquinone 6. Based on the phylogenetic and phenotypic analyses, strain HY1 should be classified as representing a novel species within the genus , for which the name sp. nov. is proposed. The type strain is HY1 (=CGMCC 1.6101=JCM 14015). Based on this study and on previously described species, an emended description of the genus is given.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64658-0
2007-02-01
2024-11-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/2/219.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64658-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  2. Bowman J. P., McCuaig R. D. 2003; Biodiversity, community structural shifts, and biogeography of prokaryotes within Antarctic continental shelf sediment. Appl Environ Microbiol 69:2463–2483 [CrossRef]
    [Google Scholar]
  3. Bowman J. P., Nichols D. S. 2005; Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp.nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia , Gelidibacter and Gillisia . Int J Syst Evol Microbiol 55:1471–1486 [CrossRef]
    [Google Scholar]
  4. Cho J.-C., Giovannoni S. J. 2003; Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α - Proteobacteria . Int J Syst Evol Microbiol 53:1031–1036 [CrossRef]
    [Google Scholar]
  5. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp  267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  6. Dobson S. J., Colwell R. R., McMeekin T. A., Franzmann P. D. 1993; Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol 43:77–83 [CrossRef]
    [Google Scholar]
  7. Ivanova E. P., Alexeeva Y. V., Flavier S., Wright J. P., Zhukova N. V., Gorshkova N. M., Mikhailov V. V., Nicolau D. V., Christen R. 2004; Formosa algae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae . Int J Syst Evol Microbiol 54:705–711 [CrossRef]
    [Google Scholar]
  8. Ivanova E. P., Bowman J. P., Christen R., Zhukova N. V., Lysenko A. M., Gorshkova N. M., Mitik-Dineva N., Sergeev A. F., Mikhailov V. V. 2006; Salegentibacter flavus sp. nov. Int J Syst Evol Microbiol 56:583–586 [CrossRef]
    [Google Scholar]
  9. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  10. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  11. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12:195–206
    [Google Scholar]
  12. McCammon S. A., Bowman J. P. 2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp.nov. and Flavobacterium xanthum sp. nov., nom.rev. and reclassification of [ Flavobacterium ] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50:1055–1063 [CrossRef]
    [Google Scholar]
  13. Nedashkovskaya O. I., Suzuki M., Vancanneyt M., Cleenwerck I., Zhukova N. V., Vysotskii M. V., Mikhailov V. V., Swings J. 2004; Salegentibacter holothuriorum sp. nov., isolated from the edible holothurian Apostichopus japonicus . Int J Syst Evol Microbiol 54:1107–1110 [CrossRef]
    [Google Scholar]
  14. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Mikhailov V. V., Bae K. S., Kim I. S. 2005; Salegentibacter mishustinae sp. nov., isolated from the sea urchin Strongylocentrotus intermedius . Int J Syst Evol Microbiol 55:235–238 [CrossRef]
    [Google Scholar]
  15. Nedashkovskaya O. I., Kim S. B., Vancanneyt M., Shin D. S., Lysenko A. M., Shevchenko L. S., Krasokhin V. B., Mikhailov V. V., Swings J., Bae K. S. 2006; Salegentibacter agarivorans sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the sponge Artemisina sp. Int J Syst Evol Microbiol 56:883–887 [CrossRef]
    [Google Scholar]
  16. Seidler R. J., Mandel M. 1971; Quantitative aspects of deoxyribonucleic acid renaturation: base composition, state of chromosome replication, and polynucleotide homologies. J Bacteriol 106:608–614
    [Google Scholar]
  17. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. 2004; Gillisia limnaea gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a microbial mat in Lake Fryxell, Antarctica. Int J Syst Evol Microbiol 54:445–448 [CrossRef]
    [Google Scholar]
  18. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989 Analysis of menaquinone compound in microbial cells by HPLC Microbiology (English translation of Mikrobiologiia ); 16176–178
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.64658-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64658-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error