A thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterial strain, designated RL80JIVT, was isolated from a geothermally active underground mine in Japan. Cells were rod-shaped and motile. The temperature and pH ranges for growth were 61–80 °C (optimum at 69–72 °C) and pH 6.4–7.9 (optimum at pH 6.8–7.3), and the strain tolerated up to 0.5 % NaCl. Strain RL80JIVT utilized sulfate, sulfite, thiosulfate and elemental sulfur as electron acceptors. Electron donors utilized were H2 in the presence of CO2, and carboxylic acids. Fermentative growth occurred on lactate and pyruvate. The cell wall contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone MK-7. Major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 0 and C16 : 0. Strain RL80JIVT was found to be affiliated with the thiosulfate-reducer Thermanaeromonas toyohensis DSM 14490T (90.9 % 16S rRNA gene sequence similarity) and with the sulfate-reducer Desulfotomaculum thermocisternum DSM 10259T (90.0 % similarity). Strain RL80JIVT is therefore considered to represent a novel species of a new genus, for which the name Desulfovirgula thermocuniculi gen. nov., sp. nov. is proposed. The type strain of Desulfovirgula thermocuniculi is RL80JIVT (=DSM 16036T=JCM 13928T).
BeederJ.,
NilsenR. K.,
RosnesJ. T.,
TorsvikT.,
LienT.1994; Archaeoglobus fulgidus isolated from hot North Sea oil field waters. Appl Environ Microbiol 60:1227–1231
CashionP.,
Holder-FranklinM. A.,
McCullyJ.,
FranklinM.1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466[CrossRef]
GoorissenH. P.,
BoschkerH. T. S.,
StamsA. J. M.,
HansenT. A.2003; Isolation of thermophilic Desulfotomaculum strains with methanol and sulfite from solfataric mud pools, and characterization of Desulfotomaculum solfataricum sp. nov. Int J Syst Evol Microbiol 53:1223–1229[CrossRef]
GrothI.,
SchumannP.,
WeissN.,
MartinK.,
RaineyF. A.1996; Agrococcus jenesis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239[CrossRef]
HenryE. A.,
DevereuxR.,
MakiJ. S.,
GilmourC. C.,
WoeseC. R.,
MandelcoL.,
SchauderR.,
RemsenC. C.,
MitchellR.1994; Characterization of a new thermophilic sulfate-reducing bacterium Thermodesulfovibrio yellowstonii , gen. nov. and sp. nov.: its phylogenetic relationship to Thermodesulfobacterium commune and their origins deep within the bacterial domain. Arch Microbiol 161:62–69[CrossRef]
KaksonenA. H.,
PlumbJ. J.,
RobertsonW. J.,
SpringS.,
SchumannP.,
FranzmannP. D.,
PuhakkaJ. A.2006a; Novel thermophilic sulfate-reducing bacteria from a geothermally active underground mine in Japan. Appl Environ Microbiol 72:3759–3762[CrossRef]
KaksonenA. H.,
SpringS.,
SchumannP.,
KroppenstedtR. M.,
PuhakkaJ. A.2006b; Desulfotomaculum thermosubterraneum sp. nov., a thermophilic sulfate-reducer isolated from an underground mine located in a geothermally active area. Int J Syst Evol Microbiol 56:2603–2608[CrossRef]
KämpferP.,
KroppenstedtR. M.1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005[CrossRef]
KarnauchowT. M.,
KovalS. F.,
JarrellK. F.1992; Isolation and characterization of 3 thermophilic anaerobes from a St. Lucia hot spring. Syst Appl Microbiol 15:296–310[CrossRef]
KroppenstedtR. M.1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp 173–179 Edited by
GoodfellowM.,
MinnikinD. E.
London: Academic Press;
LiuY.,
KarnauchowT. M.,
JarrellK. F.,
BalkwillD. L.,
DrakeG. R.,
RingelbergD.,
ClarnoR.,
BooneD. R.1997; Description of two new thermophilic Desulfotomaculum spp., Desulfotomaculum putei sp. nov., from a deep terrestrial subsurface, and Desulfotomaculum luciae sp. nov., from a hot spring. Int J Syst Bacteriol 47:615–621[CrossRef]
LoveC. A.,
PatelB. K. C.,
NicholsP. D.,
StackebrandtE.1993; Desulfotomaculum australicum , sp. nov., a thermophilic sulfate-reducing bacterium isolated from the Great Artesian Basin of Australia. Syst Appl Microbiol 16:244–251[CrossRef]
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167[CrossRef]
MonciardiniP.,
CavalettiL.,
SchumannP.,
RohdeM.,
DonadioS.2003; Conexibacter woesei gen. nov., sp. nov., a novel representative of a deep evolutionary line of descent within the class Actinobacteria
. Int J Syst Evol Microbiol 53:569–576[CrossRef]
MoriK.,
HanadaS.,
MaruyamaA.,
MarumoK.2002; Thermanaeromonas toyohensis gen. nov., sp. nov., a novel thermophilic anaerobe isolated from a subterranean vein in the Toyoha Mines. Int J Syst Evol Microbiol 52:1675–1680[CrossRef]
MoriK.,
KimH.,
KakegawaT.,
HanadaS.2003; A novel lineage of sulfate-reducing microorganisms: Thermodesulfobiaceae fam. nov., Thermodesulfobium narugense , gen. nov., sp. nov., a new thermophilic isolate from a hot spring. Extremophiles 7:283–290[CrossRef]
MoussardH.,
L'HaridonS.,
TindallB. J.,
BantaA.,
SchumannP.,
StackebrandtE.,
ReysenbachA.-L.,
JeanthonC.2004; Thermodesulfatator indicus gen. nov., sp. nov., a novel thermophilic chemolithoautotrophic sulfate-reducing bacterium isolated from the Central Indian Ridge. Int J Syst Evol Microbiol 54:227–233[CrossRef]
NilsenR. K.,
TorsvikT.,
LienT.1996; Desulfotomaculum thermocisternum sp. nov., a sulfate-reducer isolated from a hot North Sea oil reservoir. Int J Syst Bacteriol 46:397–402[CrossRef]
ReesG. N.,
GrassiaG. S.,
SheehyA. J.,
DwivediP. P.,
PatelB. K. C.1995; Desulfacinum infernum gen. nov., sp. nov., a thermophilic sulfate-reducing bacterium from a petroleum reservoir. Int J Syst Bacteriol 45:85–89[CrossRef]
RhulandL. E.,
WorkE.,
DenmanR. F.,
HoareD. S.1955; The behaviour of the isomers of α , ϵ -diaminopimelic acid on paper chromatograms. J Am Chem Soc 77:4844–4846[CrossRef]
RosnesJ. T.,
TorsvikT.,
LienT.1991; Spore-forming thermophilic sulfate-reducing bacteria isolated from North Sea oil field waters. Appl Environ Microbiol 57:2302–2307
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128[CrossRef]
ZeikusJ. G.,
DawsonM. A.,
ThompsonT. E.,
IngvorsenK.,
HatchikianE. C.1983; Microbial ecology of volcanic sulfidogenesis: isolation and characterization of Thermodesulfobacterium commune gen. nov. and sp. nov. J Gen Microbiol 129:1159–1169