1887

Abstract

Two Gram-negative, facultatively anaerobic bacterial strains, MANO22D and MANO22P, were isolated from a tidal flat area of Dae-Chun, Chung-Nam, Korea. The isolates were rod-shaped and were motile by means of one or more polar flagella. They grew at 1–12 % NaCl, 4–45 °C and pH 4.1–8.8 and were oxidase-positive, arginine dihydrolase-negative and sensitive to the vibriostatic agent O/129. The isolates required Na for growth, produced acid, but no gas, from -glucose under anaerobic conditions and utilized a wide range of compounds as sole carbon and energy sources. A phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belong to the and are specifically related to species. They were most closely related to FERM P-14531, with which they were found to share 98.65 % 16S rRNA gene sequence similarity. In the phylogenetic tree, the two novel strains comprised a relatively long subline of descent, sharing a branching point with the outlying species , and were found to occupy a phylogenetically distant position on the main branch. The levels of DNA–DNA hybridization with respect to FERM P-14531, which is their most closely related phylogenetically related species, were 7.4 % (MANO22D) and 3.9 % (MANO22P). Thus, the two novel isolates appear to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is MANO22D (=KCTC 12520=DSM 17657).

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2007-03-01
2019-10-14
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References

  1. Austin, B. ( 1988; ). Marine Microbiology. Cambridge: Cambridge University Press.
  2. Bae, J. W., Rhee, S. K., Park, J. R., Chung, W. H., Nam, Y. D., Lee, I., Kim, H. & Park, Y. H. ( 2005; ). Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria. Appl Environ Microbiol 71, 8825–8835.[CrossRef]
    [Google Scholar]
  3. Baumann, P. & Baumann, L. ( 1984; ). Genus II. Photobacterium Beijerinck 1889, 401AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 539–545. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  4. Baumann, P. & Schubert, R. H. W. ( 1984; ). Family II. Vibrionaceae Veron 1965, 5245AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 516–517. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  5. Gomez-Gil, B., Tron-Mayén, L., Roque, A., Turnbull, J. F., Inglis, V. & Guerra-Flores, A. L. ( 1998; ). Species of Vibrio isolated from hepatopancreas, haemolymph and digestive tract of a population of healthy juvenile Penaeus vannamei. Aquaculture 163, 1–9.[CrossRef]
    [Google Scholar]
  6. Gomez-Gil, B., Roque, A. & Turnbull, J. F. ( 2000; ). The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms. Aquaculture 191, 259–270.[CrossRef]
    [Google Scholar]
  7. Gomez-Gil, B., Roque, A. & Velasco-Blanco, G. ( 2002; ). Culture of Vibrio alginolyticus C7b, a potential probiotic bacterium, with the microalga Chaetoceros muelleri. Aquaculture 211, 43–48.[CrossRef]
    [Google Scholar]
  8. Gonzalez, J. M., Mayer, F., Moran, M. A., Hodson, R. E. & Whitman, W. B. ( 1997; ). Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47, 369–376.[CrossRef]
    [Google Scholar]
  9. Gordon, R. E., Haynes, W. C. & Pang, C. H.-N. ( 1973; ). The Genus Bacillus. Washington DC: United States Department of Agriculture.
  10. Hjeltnes, B. & Roberts, R. J. ( 1993; ). Vibriosis. In Bacterial Diseases of Fish, pp. 109–121. Edited by V. Inglis, R. J. Roberts & N. R. Bromage. Oxford: Blackwell Scientific.
  11. Kim, B. S., Oh, H. M., Kang, H., Park, S. S. & Chun, J. ( 2004; ). Remarkable bacterial diversity in the tidal flat sediment as revealed by 16S rDNA analysis. J Microbiol Biotechnol 14, 205–211.
    [Google Scholar]
  12. Kim, B. S., Oh, H. M., Kang, H. & Chun, J. ( 2005; ). Archaeal diversity in tidal flat sediment as revealed by 16S rDNA analysis. J Microbiol 43, 144–151.
    [Google Scholar]
  13. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  14. Lightner, D. V. ( 1993; ). Diseases of cultured penaeid shrimp. In CRC Handbook of Mariculture, 2nd edn, vol. 1, pp. 393–486. Edited by J. P. McVey. Boca Raton: FL: CRC Press.
  15. Mellado, E., Moore, E. R. B., Nieto, J. J. & Ventosa, A. ( 1996; ). Analysis of 16S rRNA gene sequences of Vibrio costicola strains: description of Salinivibrio costicola gen. nov., comb. nov. Int J Syst Bacteriol 46, 817–821.[CrossRef]
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  18. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Newark, DE: MIDI Inc.
  19. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  20. Thompson, F. L., Hoste, B., Vandemeulebroecke, K. & Swings, J. ( 2003; ). Reclassification of Vibrio hollisae as Grimontia hollisae gen. nov., comb. nov. Int J Syst Evol Microbiol 53, 1615–1617.[CrossRef]
    [Google Scholar]
  21. Thompson, F. L., Iida, T. & Swings, J. ( 2004; ). Biodiversity of vibrios. Microbiol Mol Biol Rev 68, 403–431.[CrossRef]
    [Google Scholar]
  22. Yoon, J. H., Lee, S. T. & Park, Y. H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  23. Yoon, J. H., Kang, K. H. & Park, Y. H. ( 2003; ). Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53, 687–693.[CrossRef]
    [Google Scholar]
  24. Yumoto, I. I., Iwata, H., Sawabe, T., Ueno, K., Ichise, N., Matsuyama, H., Okuyama, H. & Kawasaki, K. ( 1999; ). Characterization of a facultatively psychrophilic bacterium, Vibrio rumoiensis sp. nov., that exhibits high catalase activity. Appl Environ Microbiol 65, 67–72.
    [Google Scholar]
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