1887

Abstract

Two Gram-negative, facultatively anaerobic bacterial strains, MANO22D and MANO22P, were isolated from a tidal flat area of Dae-Chun, Chung-Nam, Korea. The isolates were rod-shaped and were motile by means of one or more polar flagella. They grew at 1–12 % NaCl, 4–45 °C and pH 4.1–8.8 and were oxidase-positive, arginine dihydrolase-negative and sensitive to the vibriostatic agent O/129. The isolates required Na for growth, produced acid, but no gas, from -glucose under anaerobic conditions and utilized a wide range of compounds as sole carbon and energy sources. A phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belong to the and are specifically related to species. They were most closely related to FERM P-14531, with which they were found to share 98.65 % 16S rRNA gene sequence similarity. In the phylogenetic tree, the two novel strains comprised a relatively long subline of descent, sharing a branching point with the outlying species , and were found to occupy a phylogenetically distant position on the main branch. The levels of DNA–DNA hybridization with respect to FERM P-14531, which is their most closely related phylogenetically related species, were 7.4 % (MANO22D) and 3.9 % (MANO22P). Thus, the two novel isolates appear to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is MANO22D (=KCTC 12520=DSM 17657).

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2007-03-01
2021-04-15
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