1887

Abstract

Five endospore-forming, nitrogen-fixing strains were isolated from rhizosphere soils of planted in Beijing, China. Phylogenetic analysis based on full-length 16S rRNA gene sequences revealed that the five strains formed a distinct cluster within the genus . High levels of 16S rRNA gene sequence similarity were found between these novel strains and ATCC 35681 (97.8–98.5 % similarity) and DSM 14472 (95.4–96.3 %). Levels of 16S rRNA gene sequence similarity between the novel isolates and other species of the genus were less than 95.0 %. Levels of 16S rRNA gene sequence similarity among the isolates were more than 98.0 %. DNA–DNA relatedness between the five novel isolates and ATCC 35681 was 45.50–47.45 % and relatedness among the five novel strains was 95.8–99.6 %. A significant feature of the novel strains that differentiated them from and other species was that none of the novel strains could produce acid or gas from the following various carbohydrates: glucose, sucrose, lactose, fructose, glycerol, xylose, maltose, -sorbitol, sodium succinate, sodium citrate, glycine or -aspartate. Anteiso-branched C was the major fatty acid component (36.59 %) of novel strain JH29. On the basis of phenotypic properties, 16S rRNA gene sequences, DNA G+C content, DNA–DNA hybridization, chemotaxonomic properties and the gene sequence, the five novel strains form a very homogeneous group which is different from other related species within the genus . Therefore, the five novel strains are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JH29 (=CCBAU 10243=DSM 18202).

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2007-04-01
2021-04-18
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