1887

Abstract

Four Gram-negative, facultatively anaerobic, non-spore-forming isolates of coccoid rods were obtained from fruit powder and investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis allocated the isolates to the family . Their phylogenetic position within the family was confirmed by sequence analysis and as the highest sequence similarities were obtained with , and , the isolates clearly belong to the genus . Biochemical data revealed that the isolates can be separated into two distinct groups that represent two novel species, as confirmed by DNA–DNA hybridizations. The two novel species can be differentiated from their nearest neighbours by the following characteristics: the utilization of sucrose, -sorbitol, putrescine and mucate, the hydrolysis of aesculin and a negative result in the Voges–Proskauer reaction. It is therefore proposed that these novel isolates are classified as sp. nov. (type strain 508/05=LMG 23730=DSM 18397) and sp. nov. (type strain 513/05=LMG 23732=DSM 18396).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64650-0
2007-04-01
2024-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/4/820.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64650-0&mimeType=html&fmt=ahah

References

  1. Drancourt M., Bollet C., Carta A., Rousselier P. 2001; Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov. Int J Syst Evol Microbiol 51:925–932 [CrossRef]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  3. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K. 1998; Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44:1148–1153 [CrossRef]
    [Google Scholar]
  4. Hoffmann H., Stindl S., Ludwig W., Stumpf A., Mehlen A., Heesemann J., Monget D., Schleifer K. H., Roggenkamp A. 2005; Reassignment of Enterobacter dissolvens to Enterobacter cloacae as E. cloacae subspecies dissolvens comb. nov. and emended description of Enterobacter asburiae and Enterobacter kobei . Syst Appl Microbiol 28:196–205 [CrossRef]
    [Google Scholar]
  5. Inoue K., Sugiyama K., Kosako Y., Sakazaki R., Yamai S. 2000; Enterobacter cowanii sp. nov., a new species of the Enterobacteriaceae . Curr Microbiol 41:417–420 [CrossRef]
    [Google Scholar]
  6. Iversen C., Druggan P., Forsythe S. 2004; A selective medium for Enterobacter sakazakii , a preliminary study. Int J Food Microbiol 96:133–139 [CrossRef]
    [Google Scholar]
  7. Kämpfer P., Ruppel S., Remus R. 2005; Enterobacter radicincitans sp. nov., a plant growth promoting species of the family Enterobacteriaceae . Syst Appl Microbiol 28:213–221 [CrossRef]
    [Google Scholar]
  8. Lehner A., Tasara T., Stephan R. 2004; 16S rRNA gene based analysis of Enterobacter sakazakii strains from different sources and development of a PCR assay for identification. BMC Microbiol 4:43 [CrossRef]
    [Google Scholar]
  9. Lehner A., Nitzsche S., Breeuwer P., Diep B., Thelen K., Stephan R. 2006; Comparison of two chromogenic media and evaluation of two molecular based identification systems for Enterobacter sakazakii detection. BMC Microbiol 6:15 [CrossRef]
    [Google Scholar]
  10. Li X., Zhang D., Chen F., Ma J., Dong Y., Zhang L. 2004; Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium. Int J Syst Evol Microbiol 54:2131–2136 [CrossRef]
    [Google Scholar]
  11. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar R., Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Mollet C., Drancourt M., Raoult D. 1997; rpoB sequence analysis as a novel basis for bacterial identification. Mol Microbiol 26:1005–1011 [CrossRef]
    [Google Scholar]
  14. Niemann S., Puhler A., Tichy H. V., Simon R., Selbitschka W. 1997; Evaluation of the resolving power of three different DNA fingerprinting methods to discriminate among isolates of a natural Rhizobium meliloti population. J Appl Microbiol 82:477–484 [CrossRef]
    [Google Scholar]
  15. Oh S. W., Kang D. H. 2004; Fluorogenic selective and differential medium for isolation of Enterobacter sakazakii . Appl Environ Microbiol 70:5692–5694 [CrossRef]
    [Google Scholar]
  16. Richard C. 1984; Genus VI. Enterobacter Hormaeche & Edwards 1960 In Bergey's Manual of Systematic Bacteriology . vol 1 pp  465–469 Edited by Staley J. T., Bryant M. P., Pfenning N., Holt J. G. Baltimore: Williams & Wilkins;
  17. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  18. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  19. Van de Peer A., De Wachter R. 1994; treecon for windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  20. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O. K., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray T. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  21. Wilson K. 1987; Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology pp. 2.4.1–2.4.5 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A, Struhl K. New York: Green Publishing and Wiley-Interscience;
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.64650-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64650-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error