1887

Abstract

To study biological phenomena in the Dead Sea and to simulate the effects of mixing Dead Sea water with Red Sea water, experimental mesocosms were operated at the Dead Sea Works at Sedom, Israel. Dense communities of red halophilic archaea developed in mesocosms filled with 80 % Dead Sea water and 20 % Red Sea water after enrichment with phosphate. The most common type of colonies isolated from these brines belonged to the genus . A few white–pinkish opaque colonies contained pleomorphic flat cells with gas vesicles. Three strains isolated from the latter colonies were characterized in depth. Their 16S rRNA gene sequences showed only 91 % similarity to the closest cultured relative (), indicating that the new strains represent a novel species of a new genus. The name gen. nov., sp. nov. is proposed for this novel organism. The type strain of is RE-101 (=DSM 17983=JCM 14081).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64648-0
2007-04-01
2019-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/4/780.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64648-0&mimeType=html&fmt=ahah

References

  1. Baker, G. C., Smith, J. J. & Cowan, D. A. ( 2003; ). Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55, 541–555.[CrossRef]
    [Google Scholar]
  2. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  3. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  4. Gutíérrez, C. & González, C. ( 1972; ). Method for simultaneous detection of proteinase and esterase activities in extremely halophilic bacteria. Appl Microbiol 24, 516–517.
    [Google Scholar]
  5. Holding, A. J. & Collee, J. G. ( 1971; ). Routine biochemical tests. Methods Microbiol 6A, 2–32.
    [Google Scholar]
  6. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  7. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  8. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  9. Martinez-Murcia, A. J., Acinas, S. G. & Rodriguez-Valera, F. ( 1995; ). Evaluation of prokaryotic diversity by restriction digestion of 16S rDNA directly amplified from hypersaline environments. FEMS Microbiol Ecol 17, 247–256.[CrossRef]
    [Google Scholar]
  10. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  11. Oren, A. ( 1999; ). Microbiological studies in the Dead Sea: future challenges toward the understanding of life at the limit of salt concentrations. Hydrobiologia 405, 1–9.[CrossRef]
    [Google Scholar]
  12. Oren, A. ( 2000; ). Biological processes in the Dead Sea as influenced by short-term and long-term salinity changes. Arch Hydrobiol Spec Issues Adv Limnol 55, 531–542.
    [Google Scholar]
  13. Oren, A. & Shilo, M. ( 1985; ). Factors determining the development of algal and bacterial blooms in the Dead Sea: a study of simulation experiments in outdoor ponds. FEMS Microbiol Ecol 31, 229–237.[CrossRef]
    [Google Scholar]
  14. Oren, A., Duker, S. & Ritter, S. ( 1996; ). The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 138, 135–140.[CrossRef]
    [Google Scholar]
  15. Oren, A., Ventosa, A. & Grant, W. D. ( 1997; ). Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47, 233–238.[CrossRef]
    [Google Scholar]
  16. Oren, A., Gavrieli, I., Gavrieli, J., Kohen, M., Lati, J. & Aharoni, M. ( 2004; ). Biological effects of dilution of Dead Sea brine with seawater: implications for the planning of the Red Sea–Dead Sea “Peace Conduit”. J Mar Syst 46, 121–131.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Sørensen, K. B., Canfield, D. E., Teske, A. P. & Oren, A. ( 2005; ). Community composition of a hypersaline endoevaporitic microbial mat. Appl Environ Microbiol 71, 7352–7365.[CrossRef]
    [Google Scholar]
  19. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  20. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignments through sequence weighing, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  21. Whitehead, T. R. & Cotta, M. A. ( 1999; ). Phylogenetic diversity of methanogenic Archaea in swine waste storage pits. FEMS Microbiol Lett 179, 223–226.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64648-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64648-0
Loading

Data & Media loading...

Supplements

vol. , part 4, pp. 780 - 783

16S rRNA gene sequence-based phylogeny reconstructed from distance values using the neighbour-joining method, including both cultured representatives of the and environmental sequences. [PDF](14 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error