RT Journal Article SR Electronic(1) A1 Nunoura, Takuro A1 Oida, Hanako A1 Miyazaki, Masayuki A1 Suzuki, Yohey A1 Takai, Ken A1 Horikoshi, KokiYR 2007 T1 Marinitoga okinawensis sp. nov., a novel thermophilic and anaerobic heterotroph isolated from a deep-sea hydrothermal field, Southern Okinawa Trough JF International Journal of Systematic and Evolutionary Microbiology, VO 57 IS 3 SP 467 OP 471 DO https://doi.org/10.1099/ijs.0.64640-0 PB Microbiology Society, SN 1466-5034, AB A novel thermophilic and sulfur-reducing heterotrophic bacterium, strain TFS10-5T, was isolated from a deep-sea hydrothermal field in Yonaguni Knoll IV, Southern Okinawa Trough. Cells of strain TFS10-5T were motile rods, 1.5–5 μm in length and 0.5–0.8 μm in width. Strain TFS10-5T was an obligately anaerobic heterotroph and sulfur-reduction stimulated growth. Growth was observed between 30 and 70 °C (optimum at 55–60 °C), pH 5.0–7.4 (optimum at pH 5.5–5.8), 1.0–5.5 NaCl % (optimum at 3.0–3.5 %). The fatty acid content was C16 : 0 (71.0 %), C16 : 1 (6.0 %), C18 : 0 (21.4 %) and C18 : 1 (1.6 %). The G+C content of the genomic DNA was 28 mol%. 16S rRNA gene sequence analysis indicated that strain TFS10-5T belongs to the genus Marinitoga. Based on the physiological and phylogenetic features of the new isolate, strain TFS10-5T represents a novel species in the genus Marinitoga for which the name Marinitoga okinawensis sp. nov. is proposed. The type strain is TFS10-5T (=JCM 13303T=DSM 17373T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64640-0