1887

Abstract

A novel thermophilic and sulfur-reducing heterotrophic bacterium, strain TFS10-5, was isolated from a deep-sea hydrothermal field in Yonaguni Knoll IV, Southern Okinawa Trough. Cells of strain TFS10-5 were motile rods, 1.5–5 μm in length and 0.5–0.8 μm in width. Strain TFS10-5 was an obligately anaerobic heterotroph and sulfur-reduction stimulated growth. Growth was observed between 30 and 70 °C (optimum at 55–60 °C), pH 5.0–7.4 (optimum at pH 5.5–5.8), 1.0–5.5 NaCl % (optimum at 3.0–3.5 %). The fatty acid content was C (71.0 %), C (6.0 %), C (21.4 %) and C (1.6 %). The G+C content of the genomic DNA was 28 mol%. 16S rRNA gene sequence analysis indicated that strain TFS10-5 belongs to the genus . Based on the physiological and phylogenetic features of the new isolate, strain TFS10-5 represents a novel species in the genus for which the name sp. nov. is proposed. The type strain is TFS10-5 (=JCM 13303=DSM 17373).

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2007-03-01
2020-09-29
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References

  1. Alain K., Marteinsson V. T., Miroshnichenko M. L., Bonch-Osmolovskaya E. A., Prieur D., Birrien J.-L. 2002; Marinitoga piezophila sp. nov., a rod-shaped thermo-piezophilic bacterium isolated under high hydrostatic pressure from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 52:1331–1339 [CrossRef]
    [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. 1979; Methanogens: reevaluation of a unique biological group. Microbiol Rev 43:260–296
    [Google Scholar]
  3. Charlou J. L., Fouquet Y., Donval J. P., Auzende J. M., Jean-Baptiste P., Stievenard M., Michel S. 1996; Mineral and gas chemistry of hydrothermal fluids on an ultrafast spreading ridge: East Pacific Rise, 17° to 19° S (Naudur cruise, 1993) phase separation processes controlled by volcanic and tectonic activity. J Geophys Res 101:15899–15919 [CrossRef]
    [Google Scholar]
  4. Charlou J. L., Donval J. P., Douville E., Jean-Baptiste P., Radford-Knoery J., Fouquet Y., Dapoigny A., Stievenard M. (2000); Compared geochemical signatures and the evolution of Menez Gwen (37° 50′ N) and Lucky Strike (37° 17′ N) hydrothermal fluids, south of the Azores Triple Junction on the Mid-Atlantic Ridge. Chem Geol 171:49–75 [CrossRef]
    [Google Scholar]
  5. Davey M. E., Wood W. A., Key R., Nakamura K., Stahl D. 1993; Isolation of three species of Geotoga and Petrotoga : two new genera, representing a new lineage in the bacterial line of descent distantly related to the ‘ Thermotogales ’. Syst Appl Microbiol 16:191–200 [CrossRef]
    [Google Scholar]
  6. DeLong E. F. 1992; Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89:5685–5689 [CrossRef]
    [Google Scholar]
  7. Donval J. P., Charlou J. L., Douville E., Knoery J., Fouquet Y., Poncevera E., Jean-Baptiste P., Stievenard M., German C. R. other authors 1997; High H2 and CH4 content in hydrothermal fluids from Rainbow site newly sampled at 36° 14′ N on the AMAR segment, Mid-Atlantic Ridge (diving FLORES cruise, July 1997): comparison with other MAR sites. Trans Am Geophys Union 78, Supplement, F832 (abstract).
    [Google Scholar]
  8. Huber R., Langworthy T. A., König H., Thomm M., Woese C. R., Sleytr U. B., Stetter K. O. 1986; Thermotoga maritima sp. nov. represents a new genus of uniquely extremely thermophilic eubacteria growing up to 90 °C. Arch Microbiol 144:324–333 [CrossRef]
    [Google Scholar]
  9. Huber R., Woese C. R., Langworthy T. A., Fricke H., Stetter K. O. 1989; Thermosipho africanus gen. nov., represents a new genus of thermophilic eubacteria within the “ Thermotogales ”. Syst Appl Microbiol 12:32–37 [CrossRef]
    [Google Scholar]
  10. Konno U., Tsunogai U., Nakagawa F., Nakashima M., Ishibashi J., Nunoura T., Nakamura K. 2006; Liquid CO2 venting on seafloor: Yonaguni Knoll IV hydrothermal system, Okinawa Trough. Geophys Res Lett 33:L16607 [CrossRef] [CrossRef]
    [Google Scholar]
  11. Lane D. J. 1991; 16S-23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  12. Lauerer G., Kristjansson J. K., Langworthy T. A., König H., Stetter K. O. 1986; Methanothermus sociabilis sp. nov., a second species within the Methanothermaceae growing at 97 °C. Syst Appl Microbiol 8:100–105 [CrossRef]
    [Google Scholar]
  13. Patel B. K. C., Morgan H. W., Daniel R. M. 1985; Fervidobacterium nodosum gen. nov. and spec. nov., a new chemoorganotrophic, caldoactive, anaerobic bacterium. Arch Microbiol 141:63–69 [CrossRef]
    [Google Scholar]
  14. Porter K. G., Feig Y. S. 1980; The use of DAPI for identifying and counting aquatic microflora. Limnol Oceanogr 25:943–948 [CrossRef]
    [Google Scholar]
  15. Postec A., Le Breton C., Fardeau M. L., Lesongeur F., Pignet P., Querellou J., Ollivier B., Godfroy A. 2005; Marinitoga hydrogenitolerans sp. nov., a novel member of the order Thermotogales isolated from a black smoker chimney on the Mid-Atlantic Ridge. Int J Syst Evol Microbiol 55:1217–1221 [CrossRef]
    [Google Scholar]
  16. Sako Y., Takai K., Ishida Y., Uchida A., Katayama Y. 1996; Rhodothermus obamensis sp. nov., a modern lineage of extremely thermophilic marine bacteria. Int J Syst Bacteriol 46:1099–1104 [CrossRef]
    [Google Scholar]
  17. Takai K., Sugai A., Itoh T., Horikoshi K. 2000; Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney. Int J Syst Evol Microbiol 50:489–500 [CrossRef]
    [Google Scholar]
  18. Takai K., Komatsu T., Inagaki F., Horikoshi K. 2001; Distribution of archaea in a black smoker chimney structure. Appl Environ Microbiol 67:3618–3629 [CrossRef]
    [Google Scholar]
  19. Takai K., Nakagawa S., Sako Y., Horikoshi K. 2003; Balnearium lithotrophicum gen. nov., sp. nov., a novel thermophilic, strictly anaerobic, hydrogen-oxidizing chemolithoautotroph isolated from a black smoker chimney in the Suiyo Seamount hydrothermal system. Int J Syst Evol Microbiol 53:1947–1954 [CrossRef]
    [Google Scholar]
  20. Takai K., Hirayama H., Nakagawa T., Suzuki Y., Nealson K. H., Horikoshi K. 2005; Lebetimonas acidiphila gen. nov., sp. nov., a novel thermophilic, acidophilic, hydrogen-oxidizing chemolithoautotroph within the ‘Epsilonproteobacteria’, isolated from a deep-sea hydrothermal fumarole in the Mariana Arc. Int J Syst Evol Microbiol 55:183–189 [CrossRef]
    [Google Scholar]
  21. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  22. Wery N., Lesongeur F., Pignet P., Derennes V., Cambon-Bonavita M. A., Godfroy A., Barbier G. 2001; Marinitoga camini gen. nov., sp. nov., a rod-shaped bacterium belonging to the order Thermotogales , isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51:495–504
    [Google Scholar]
  23. Zillig W., Holz I., Janekovic D., Klenk H. P., Imsel E., Trent J., Wunderl S., Forjaz V. H., Coutinho R. other authors 1990; Hyperthermus butylicus , a hyperthermophilic sulfur-reducing archaebacterium that ferments peptides. J Bacteriol 172:3959–3965
    [Google Scholar]
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