1887

Abstract

A novel thermophilic and sulfur-reducing heterotrophic bacterium, strain TFS10-5, was isolated from a deep-sea hydrothermal field in Yonaguni Knoll IV, Southern Okinawa Trough. Cells of strain TFS10-5 were motile rods, 1.5–5 μm in length and 0.5–0.8 μm in width. Strain TFS10-5 was an obligately anaerobic heterotroph and sulfur-reduction stimulated growth. Growth was observed between 30 and 70 °C (optimum at 55–60 °C), pH 5.0–7.4 (optimum at pH 5.5–5.8), 1.0–5.5 NaCl % (optimum at 3.0–3.5 %). The fatty acid content was C (71.0 %), C (6.0 %), C (21.4 %) and C (1.6 %). The G+C content of the genomic DNA was 28 mol%. 16S rRNA gene sequence analysis indicated that strain TFS10-5 belongs to the genus . Based on the physiological and phylogenetic features of the new isolate, strain TFS10-5 represents a novel species in the genus for which the name sp. nov. is proposed. The type strain is TFS10-5 (=JCM 13303=DSM 17373).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64640-0
2007-03-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/3/467.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64640-0&mimeType=html&fmt=ahah

References

  1. Alain, K., Marteinsson, V. T., Miroshnichenko, M. L., Bonch-Osmolovskaya, E. A., Prieur, D. & Birrien, J.-L. ( 2002; ). Marinitoga piezophila sp. nov., a rod-shaped thermo-piezophilic bacterium isolated under high hydrostatic pressure from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 52, 1331–1339.[CrossRef]
    [Google Scholar]
  2. Balch, W. E., Fox, G. E., Magrum, L. J., Woese, C. R. & Wolfe, R. S. ( 1979; ). Methanogens: reevaluation of a unique biological group. Microbiol Rev 43, 260–296.
    [Google Scholar]
  3. Charlou, J. L., Fouquet, Y., Donval, J. P., Auzende, J. M., Jean-Baptiste, P., Stievenard, M. & Michel, S. ( 1996; ). Mineral and gas chemistry of hydrothermal fluids on an ultrafast spreading ridge: East Pacific Rise, 17° to 19° S (Naudur cruise, 1993) phase separation processes controlled by volcanic and tectonic activity. J Geophys Res 101, 15899–15919.[CrossRef]
    [Google Scholar]
  4. Charlou, J. L., Donval, J. P., Douville, E., Jean-Baptiste, P., Radford-Knoery, J., Fouquet, Y., Dapoigny, A. & Stievenard, M. ( 2000; ). Compared geochemical signatures and the evolution of Menez Gwen (37° 50′ N) and Lucky Strike (37° 17′ N) hydrothermal fluids, south of the Azores Triple Junction on the Mid-Atlantic Ridge. Chem Geol 171, 49–75.[CrossRef]
    [Google Scholar]
  5. Davey, M. E., Wood, W. A., Key, R., Nakamura, K. & Stahl, D. ( 1993; ). Isolation of three species of Geotoga and Petrotoga: two new genera, representing a new lineage in the bacterial line of descent distantly related to the ‘Thermotogales’. Syst Appl Microbiol 16, 191–200.[CrossRef]
    [Google Scholar]
  6. DeLong, E. F. ( 1992; ). Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89, 5685–5689.[CrossRef]
    [Google Scholar]
  7. Donval, J. P., Charlou, J. L., Douville, E., Knoery, J., Fouquet, Y., Poncevera, E., Jean-Baptiste, P., Stievenard, M., German, C. R. & other authors ( 1997; ). High H2 and CH4 content in hydrothermal fluids from Rainbow site newly sampled at 36° 14′ N on the AMAR segment, Mid-Atlantic Ridge (diving FLORES cruise, July 1997): comparison with other MAR sites. Trans Am Geophys Union 78, Supplement, F832 (abstract).
    [Google Scholar]
  8. Huber, R., Langworthy, T. A., König, H., Thomm, M., Woese, C. R., Sleytr, U. B. & Stetter, K. O. ( 1986; ). Thermotoga maritima sp. nov. represents a new genus of uniquely extremely thermophilic eubacteria growing up to 90 °C. Arch Microbiol 144, 324–333.[CrossRef]
    [Google Scholar]
  9. Huber, R., Woese, C. R., Langworthy, T. A., Fricke, H. & Stetter, K. O. ( 1989; ). Thermosipho africanus gen. nov., represents a new genus of thermophilic eubacteria within the “Thermotogales”. Syst Appl Microbiol 12, 32–37.[CrossRef]
    [Google Scholar]
  10. Konno, U., Tsunogai, U., Nakagawa, F., Nakashima, M., Ishibashi, J., Nunoura, T. & Nakamura, K. ( 2006; ). Liquid CO2 venting on seafloor: Yonaguni Knoll IV hydrothermal system, Okinawa Trough. Geophys Res Lett 33, L16607. doi:10.1029/2006GL026115[CrossRef]
    [Google Scholar]
  11. Lane, D. J. ( 1991; ). 16S-23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  12. Lauerer, G., Kristjansson, J. K., Langworthy, T. A., König, H. & Stetter, K. O. ( 1986; ). Methanothermus sociabilis sp. nov., a second species within the Methanothermaceae growing at 97 °C. Syst Appl Microbiol 8, 100–105.[CrossRef]
    [Google Scholar]
  13. Patel, B. K. C., Morgan, H. W. & Daniel, R. M. ( 1985; ). Fervidobacterium nodosum gen. nov. and spec. nov., a new chemoorganotrophic, caldoactive, anaerobic bacterium. Arch Microbiol 141, 63–69.[CrossRef]
    [Google Scholar]
  14. Porter, K. G. & Feig, Y. S. ( 1980; ). The use of DAPI for identifying and counting aquatic microflora. Limnol Oceanogr 25, 943–948.[CrossRef]
    [Google Scholar]
  15. Postec, A., Le Breton, C., Fardeau, M. L., Lesongeur, F., Pignet, P., Querellou, J., Ollivier, B. & Godfroy, A. ( 2005; ). Marinitoga hydrogenitolerans sp. nov., a novel member of the order Thermotogales isolated from a black smoker chimney on the Mid-Atlantic Ridge. Int J Syst Evol Microbiol 55, 1217–1221.[CrossRef]
    [Google Scholar]
  16. Sako, Y., Takai, K., Ishida, Y., Uchida, A. & Katayama, Y. ( 1996; ). Rhodothermus obamensis sp. nov., a modern lineage of extremely thermophilic marine bacteria. Int J Syst Bacteriol 46, 1099–1104.[CrossRef]
    [Google Scholar]
  17. Takai, K., Sugai, A., Itoh, T. & Horikoshi, K. ( 2000; ). Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney. Int J Syst Evol Microbiol 50, 489–500.[CrossRef]
    [Google Scholar]
  18. Takai, K., Komatsu, T., Inagaki, F. & Horikoshi, K. ( 2001; ). Distribution of archaea in a black smoker chimney structure. Appl Environ Microbiol 67, 3618–3629.[CrossRef]
    [Google Scholar]
  19. Takai, K., Nakagawa, S., Sako, Y. & Horikoshi, K. ( 2003; ). Balnearium lithotrophicum gen. nov., sp. nov., a novel thermophilic, strictly anaerobic, hydrogen-oxidizing chemolithoautotroph isolated from a black smoker chimney in the Suiyo Seamount hydrothermal system. Int J Syst Evol Microbiol 53, 1947–1954.[CrossRef]
    [Google Scholar]
  20. Takai, K., Hirayama, H., Nakagawa, T., Suzuki, Y., Nealson, K. H. & Horikoshi, K. ( 2005; ). Lebetimonas acidiphila gen. nov., sp. nov., a novel thermophilic, acidophilic, hydrogen-oxidizing chemolithoautotroph within the ‘Epsilonproteobacteria’, isolated from a deep-sea hydrothermal fumarole in the Mariana Arc. Int J Syst Evol Microbiol 55, 183–189.[CrossRef]
    [Google Scholar]
  21. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  22. Wery, N., Lesongeur, F., Pignet, P., Derennes, V., Cambon-Bonavita, M. A., Godfroy, A. & Barbier, G. ( 2001; ). Marinitoga camini gen. nov., sp. nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51, 495–504.
    [Google Scholar]
  23. Zillig, W., Holz, I., Janekovic, D., Klenk, H. P., Imsel, E., Trent, J., Wunderl, S., Forjaz, V. H., Coutinho, R. & other authors ( 1990; ). Hyperthermus butylicus, a hyperthermophilic sulfur-reducing archaebacterium that ferments peptides. J Bacteriol 172, 3959–3965.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64640-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64640-0
Loading

Data & Media loading...

Supplements

vol. , part 3, pp. 467 - 471

Graphs showing the effects of temperature, pH and NaCl concentration of the growth of strain TFS10-5 . [PDF](59 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error