1887

Abstract

A Gram-positive, coccoid, non-spore-forming bacterium, designated strain 3-6, was isolated from farmland soil and subjected to a polyphasic taxonomic analysis. Comparative analysis of the 16S rRNA gene sequence revealed that the strain represented a novel member of the suborder . Its nearest phylogenetic neighbour was the type strain of (94.2 % 16S rRNA gene sequence similarity). Chemotaxonomic characteristics of strain 3-6 were as follows: the major menaquinone was MK-8(H); the polar lipids consisted mainly of diphosphatidylglycerol, phosphatidylglycerol and one unknown glycolipid; the predominant fatty acids were anteiso-C, anteiso-C and iso-C; mycolic acids were absent. A new murein type, -lys–gly–-glu–-Glu (A4), was found in the peptidoglycan of the cell wall. The DNA G+C content was 69.8 mol%. On the basis of morphological, chemotaxonomic and phylogenetic characteristics, it is suggested that strain 3-6 represents a novel species of a new genus within the suborder , for which the name gen. nov., sp. nov. is proposed. The type strain of is 3-6 (=CGMCC 4.3142=DSM 18029=JCM 13910=PCM 2644).

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2007-04-01
2020-01-20
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Scanning electron micrograph of cells of strain 3-6 grown on R agar for 5 days at 28 °C. Bar, 5.0 µm.

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Phylogenetic analysis based on 16S rRNA gene sequences available from the GenBank/EMBL/DDBJ databases, showing the position of strain 3-6 . [PDF](17 KB)

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[PDF file of Supplementary Tables S1 and S2](111 KB)

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