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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 57, Issue 3

sp. nov., a novel type II methanotrophic bacterium possessing ‘signature’ fatty acids of type I methanotrophs Free

Abstract

A novel species is proposed for two strains of methanotrophic bacteria (H2 and Sakb1) isolated from an acidic (pH 4.3) peat bog lake (Teufelssee, Germany) and an acidic (pH 4.2) tropical forest soil (Thailand), respectively. Cells of strains H2 and Sakb1 were aerobic, Gram-negative, non-motile, straight or curved rods that were covered by large polysaccharide capsules and contained an intracytoplasmic membrane system typical of type II methanotrophs. They possessed both a particulate and a soluble methane monooxygenase and utilized the serine pathway for carbon assimilation. They were moderately acidophilic organisms capable of growth between pH 4.4 and 7.5 (optimum 5.8–6.2). The most unique characteristic of these strains was the phospholipid fatty acid profile. In addition to the signature fatty acid of type II methanotrophs (18 : 18), the cells also contained large amounts of what was previously considered to be a signature fatty acid of type I methanotrophs, 16 : 18. The DNA G+C contents of strains H2 and Sakb1 were 61.5 and 62.1 mol%, respectively. The 16S rRNA gene sequences possessed 96–98 % similarity to sequences of other type II methanotrophs in the genera and . 16S rRNA gene sequence and phylogeny demonstrated that the strains form a novel lineage within the genus . DNA–DNA hybridization values of strain H2 with OBBP and IMET 10491 were 18 and 25 %, respectively. Thus, it is proposed that these two strains represent a novel species, sp. nov. Strain H2 (=DSM 16984=VKM B-2426) is the type strain.

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Keyword(s): DMDS, dimethyldisulfide , FAME, fatty acid methyl ester , ICM, intracytoplasmic membrane , MDH, methanol dehydrogenase , PLFA, phospholipid fatty acid , pMMO, particulate methane monooxygenase and sMMO, soluble methane monooxygenase
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2007-03-01
2025-01-25
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Methylocystis heyeri sp. nov., a novel type II methanotrophic bacterium possessing ‘signature’ fatty acids of type I methanotrophs
Int J Syst Evol Microbiol 57, 472 (2007); https://doi.org/10.1099/ijs.0.64623-0
/content/journal/ijsem/10.1099/ijs.0.64623-0
/content/journal/ijsem/10.1099/ijs.0.64623-0
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