1887

Abstract

A novel species is proposed for two strains of methanotrophic bacteria (H2 and Sakb1) isolated from an acidic (pH 4.3) peat bog lake (Teufelssee, Germany) and an acidic (pH 4.2) tropical forest soil (Thailand), respectively. Cells of strains H2 and Sakb1 were aerobic, Gram-negative, non-motile, straight or curved rods that were covered by large polysaccharide capsules and contained an intracytoplasmic membrane system typical of type II methanotrophs. They possessed both a particulate and a soluble methane monooxygenase and utilized the serine pathway for carbon assimilation. They were moderately acidophilic organisms capable of growth between pH 4.4 and 7.5 (optimum 5.8–6.2). The most unique characteristic of these strains was the phospholipid fatty acid profile. In addition to the signature fatty acid of type II methanotrophs (18 : 18), the cells also contained large amounts of what was previously considered to be a signature fatty acid of type I methanotrophs, 16 : 18. The DNA G+C contents of strains H2 and Sakb1 were 61.5 and 62.1 mol%, respectively. The 16S rRNA gene sequences possessed 96–98 % similarity to sequences of other type II methanotrophs in the genera and . 16S rRNA gene sequence and phylogeny demonstrated that the strains form a novel lineage within the genus . DNA–DNA hybridization values of strain H2 with OBBP and IMET 10491 were 18 and 25 %, respectively. Thus, it is proposed that these two strains represent a novel species, sp. nov. Strain H2 (=DSM 16984=VKM B-2426) is the type strain.

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2007-03-01
2019-10-19
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References

  1. Boschker, H. T. S., Nold, S. C., Wellsbury, P., Bos, D., de Graaf, W., Pel, R., Parkes, R. J. & Cappenberg, T. E. ( 1998; ). Direct linking of microbial populations to specific biogeochemical processes by 13C-labelling of biomarkers. Nature 392, 801–805.[CrossRef]
    [Google Scholar]
  2. Boschker, H. T. S., de Graaf, W., Koster, M., Meyer-Reil, L. A. & Cappenberg, T. E. ( 2001; ). Bacterial populations and processes involved in acetate and propionate consumption in anoxic brackish sediment. FEMS Microbiol Ecol 35, 97–103.[CrossRef]
    [Google Scholar]
  3. Bowman, J. P., Sly, L. I., Nichols, P. D. & Hayward, A. C. ( 1993; ). Revised taxonomy of the methanotrophs: description of Methylobacter gen. nov., emendation of Methylococcus, validation of Methylosinus and Methylocystis species, and a proposal that the family Methylococcaceae includes only the group I methanotrophs. Int J Syst Bacteriol 43, 735–753.[CrossRef]
    [Google Scholar]
  4. Dedysh, S. N., Panikov, N. S. & Tiedje, J. M. ( 1998; ). Acidophilic methanotrophic communities from Sphagnum peat bogs. Appl Environ Microbiol 64, 922–929.
    [Google Scholar]
  5. Dedysh, S. N., Liesack, W., Khmelenina, V. N., Suzina, N. E., Trotsenko, Y. A., Semrau, J. D., Bares, A. M., Panikov, N. S. & Tiedje, J. M. ( 2000; ). Methylocella palustris gen. nov., sp. nov., a new methane-oxidizing acidophilic bacterium from peat bogs, representing a novel subtype of serine-pathway methanotrophs. Int J Syst Evol Microbiol 50, 955–969.[CrossRef]
    [Google Scholar]
  6. Dedysh, S. N., Derakshani, M. & Liesack, W. ( 2001; ). Detection and enumeration of methanotrophs in acidic Sphagnum peat by 16S rRNA fluorescence in situ hybridization, including the use of newly developed oligonucleotide probes for Methylocella palustris. Appl Environ Microbiol 67, 4850–4857.[CrossRef]
    [Google Scholar]
  7. Dedysh, S. N., Khmelenina, V. N., Suzina, N. E., Trotsenko, Y. A., Semrau, J. D., Liesack, W. & Tiedje, J. M. ( 2002; ). Methylocapsa acidiphila gen. nov., sp. nov., a novel methane-oxidizing and dinitrogen-fixing acidophilic bacterium from Sphagnum bog. Int J Syst Evol Microbiol 52, 251–261.
    [Google Scholar]
  8. Dedysh, S. N., Dunfield, P. F., Derakshani, M., Stubner, S., Heyer, J. & Liesack, W. ( 2003; ). Differential detection of type II methanotrophic bacteria in acidic peatlands using newly developed 16S rRNA-targeted fluorescent oligonucleotide probes. FEMS Microbiol Ecol 43, 299–308.[CrossRef]
    [Google Scholar]
  9. Dedysh, S. N., Berestovskaya, Y. Y., Vasylieva, L. V., Belova, S. E., Khmelenina, V. N., Suzina, N. E., Trotsenko, Y. A., Liesack, W. & Zavarzin, G. A. ( 2004; ). Methylocella tundrae sp. nov., a novel methanotrophic bacterium from acidic tundra peatlands. Int J Syst Evol Microbiol 54, 151–156.[CrossRef]
    [Google Scholar]
  10. Dunfield, P. F., Khmelenina, V. N., Suzina, N. E., Trotsenko, Y. A. & Dedysh, S. N. ( 2003; ). Methylocella silvestris sp. nov., a novel methanotroph isolated from an acidic forest cambisol. Int J Syst Evol Microbiol 53, 1231–1239.[CrossRef]
    [Google Scholar]
  11. Guckert, J. B., Antworth, C. P., Nichols, P. D. & White, D. C. ( 1985; ). Phospholipid, ester-linked fatty acid profiles as reproducible assays for changes in prokaryotic community structure of estuarine sediments. FEMS Microbiol Ecol 31, 147–158.[CrossRef]
    [Google Scholar]
  12. Guckert, J. B., Ringelberg, D. B., White, D. C., Hanson, R. S. & Bratina, B. J. ( 1991; ). Membrane fatty acids as phenotypic markers in the polyphasic taxonomy of methylotrophs within the Proteobacteria. J Gen Microbiol 137, 2631–2641.[CrossRef]
    [Google Scholar]
  13. Hanson, R. S. & Hanson, T. E. ( 1996; ). Methanotrophic bacteria. Microbiol Rev 60, 439–471.
    [Google Scholar]
  14. Heyer, J., Galchenko, V. F. & Dunfield, P. F. ( 2002; ). Molecular phylogeny of type II methane-oxidizing bacteria isolated from various environments. Microbiology 148, 2831–2846.
    [Google Scholar]
  15. Knief, C., Vanitchung, S., Harvey, N. W., Conrad, R., Dunfield, P. F. & Chidthaisong, A. ( 2005; ). Diversity of methanotrophic bacteria in tropical upland soils under different land uses. Appl Environ Microbiol 71, 3826–3831.[CrossRef]
    [Google Scholar]
  16. Krumholz, L. R., Hollenback, J. L., Roskes, S. J. & Ringelberg, D. B. ( 1995; ). Methanogenesis and methanotrophy within a Sphagnum peatland. FEMS Microbiol Ecol 18, 215–224.[CrossRef]
    [Google Scholar]
  17. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  18. Lysenko, A. M., Gal'chenko, V. F. & Chernykh, N. A. ( 1988; ). Taxonomic study of obligate methanotrophic bacteria using the DNA–DNA hybridization technique. Microbiology (English translation of Mikrobiologiia) 57, 653–658.
    [Google Scholar]
  19. McDonald, I. R. & Murrell, J. C. ( 1997; ). The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs. FEMS Microbiol Lett 156, 205–210.[CrossRef]
    [Google Scholar]
  20. Nichols, P. D., Guckert, J. B. & White, D. C. ( 1986; ). Determination of monounsaturated fatty acid double bond position and geometry for microbial monocultures and complex consortia by capillary GC-MS of their dimethyldisulfur adducts. J Microbiol Methods 5, 49–55.[CrossRef]
    [Google Scholar]
  21. Owen, R. J., Hill, L. R. & Lapage, S. P. ( 1969; ). Determination of DNA base composition from melting profiles in dilute buffers. Biopolymers 7, 503–516.[CrossRef]
    [Google Scholar]
  22. Radajewski, S., Webster, G., Reay, D. S., Morris, S. A., Ineson, P., Nedwell, D. B., Prosser, J. I. & Murrell, J. C. ( 2002; ). Identification of active methylotroph populations in an acidic forest soil by stable-isotope probing. Microbiology 148, 2331–2342.
    [Google Scholar]
  23. Sundh, I., Borga, P., Nilsson, M. & Svensson, B. H. ( 1995; ). Estimation of cell numbers of methanotrophic bacteria in boreal peatlands based on analysis of specific phospholipid fatty acids. FEMS Microbiol Ecol 18, 103–112.[CrossRef]
    [Google Scholar]
  24. Trotsenko, Y. A. & Khmelenina, V. N. ( 2002; ). Biology of extremophilic and extremotolerant methanotrophs. Arch Microbiol 177, 123–131.[CrossRef]
    [Google Scholar]
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vol. , part 3, pp. 472 - 479

Mass spectra of DMDS adducts of 16:1 PLFA of strain H2 . [PDF](76 KB)



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