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Abstract

An orange-coloured bacterial strain, designated R2A15-11, was isolated from greenhouse soil. The strain was found to be strictly aerobic, Gram-negative, non-spore-forming and non-flagellated. The cells were short rods (0.7–0.9×1.0–1.5 μm) and produced flexirubin. Growth of the strain was observed at 10–35 °C, pH 5.0–8.0 and 0–3 % (w/v) NaCl. The predominant isoprenoid quinone was MK-7. The major fatty acids were iso-C, iso-C G, iso-C 3-OH and summed feature 3 (comprising iso-C 2-OH and/or C 7). The genomic DNA G+C content was 45.0 mol%. Phylogenetic analysis of the 16S rRNA gene sequence of strain R2A15-11 revealed a clear affiliation with the phylum , and the highest levels of sequence similarity were found with respect to ATCC 13524 (91.5 %), DY (90.2 %), GR20-13 (89.9 %) and GR20-10 (89.7 %). On the basis of the polyphasic evidence from this study, strain R2A15-11 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is R2A15-11 (=KACC 11698=DSM 17617).

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2007-03-01
2020-01-24
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References

  1. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  2. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  3. Kwon, S. W., Kim, J. S., Park, I. C., Yoon, S. H., Park, D. H., Lim, C. K. & Go, S. J. ( 2003; ). Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53, 21–27.[CrossRef]
    [Google Scholar]
  4. Liu, W. T., Nielsen, A. T., Wu, J. H., Tsai, C. S., Matsuo, Y. & Molin, S. ( 2001; ). In situ identification of polyphosphate- and polyhydroxyalkanoate-accumulating traits for microbial populations in a biological phosphorus removal process. Environ Microbiol 3, 110–122.[CrossRef]
    [Google Scholar]
  5. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  6. Nakagawa, Y. & Yamasato, K. ( 1993; ). Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139, 1155–1161.[CrossRef]
    [Google Scholar]
  7. Nakagawa, Y., Sakane, T., Suzuki, M. & Hatano, K. ( 2002; ). Phylogenetic structure of the genera Flexibacter, Flexithrix, and Microscilla deduced from 16S rRNA sequence analysis. J Gen Appl Microbiol 48, 155–165.[CrossRef]
    [Google Scholar]
  8. Reichenbach, H. ( 1992; ). The order Cytophagales. In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  9. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 1–6.
    [Google Scholar]
  10. Sly, L. I., Taghavi, M. & Fegan, M. ( 1999; ). Phylogenetic position of Chitinophaga pinensis in the Flexibacter–Bacteroides–Cytophaga phylum. Int J Syst Bacteriol 49, 479–481.[CrossRef]
    [Google Scholar]
  11. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  12. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  13. Weon, H. Y., Kim, B.-Y., Yoo, S.-H., Lee, S.-Y., Kwon, S.-W., Go, S.-J. & Stackebrandt, E. ( 2006; ). Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov., novel members of the phylum Bacteroidetes, isolated from soil cultivated with Korean ginseng. Int J Syst Evol Microbiol 56, 1777–1782.[CrossRef]
    [Google Scholar]
  14. Xie, C. H. & Yokota, A. ( 2006; ). Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 1117–1121.[CrossRef]
    [Google Scholar]
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vol. , part 3, pp. 538 - 541

Cellular fatty acid compositions of strain R2A15-11 and related strains. [PDF](23 KB)



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