1887

Abstract

A biodiversity study on lactic acid bacteria (LAB) occurring in traditional Belgian sourdoughs resulted in the isolation of two isolates, LMG 23583 and LMG 23584, that could not be assigned to any recognized LAB species. The two isolates were initially investigated by means of phenylalanyl-tRNA synthase () gene sequence analysis and were found to occupy a separate position relative to recognized species present in the database. Subsequently, their phylogenetic affiliation was determined by 16S rRNA gene sequence analysis, indicating that the two isolates belong to the species group with , and as closest relatives. Whole-cell protein analysis (SDS-PAGE) and amplified fragment length polymorphism fingerprinting of whole genomes confirmed their separate taxonomic status. DNA–DNA hybridization experiments, DNA G+C content, growth characteristics and biochemical features demonstrated that the two isolates represent a novel species, for which the name sp. nov. is proposed. The type strain is LMG 23583 (=CCUG 52843).

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2007-02-01
2019-10-23
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vol. , part 2, pp. 223 - 227

Cluster analysis of digitized protein profiles of strains of sp. nov. and closest phylogenetic relatives. [PDF](22 KB)



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