RT Journal Article SR Electronic(1) A1 Suzuki, Daisuke A1 Ueki, Atsuko A1 Amaishi, Aya A1 Ueki, KatsujiYR 2007 T1 Desulfopila aestuarii gen. nov., sp. nov., a Gram-negative, rod-like, sulfate-reducing bacterium isolated from an estuarine sediment in Japan JF International Journal of Systematic and Evolutionary Microbiology, VO 57 IS 3 SP 520 OP 526 DO https://doi.org/10.1099/ijs.0.64600-0 PB Microbiology Society, SN 1466-5034, AB A strictly anaerobic, mesophilic, sulfate-reducing bacterial strain (MSL86T) isolated from an estuarine sediment in the Sea of Japan (around the Japanese islands) was characterized phenotypically and phylogenetically. The cells were found to be Gram-negative, motile, non-spore-forming rods. Catalase was not detected. The optimum NaCl concentration for growth was 1.0 % (w/v) and the optimum temperature was 35 °C. Strain MSL86T was slightly alkaliphilic, with optimum growth at pH 7.5–7.6. Organic electron donors were incompletely oxidized to (mainly) acetate. Strain MSL86T utilized formate, pyruvate, lactate, fumarate, ethanol, propanol, butanol and glycerol as electron donors for sulfate reduction and did not use acetate, propionate, butyrate, succinate, malate, methanol, glycine, alanine, serine, aspartate, glutamate or H2. Sulfite, thiosulfate and fumarate were used as electron acceptors with lactate as an electron donor. Without electron acceptors, the strain fermented pyruvate and fumarate. The genomic DNA G+C content was 54.4 mol%. Menaquinone MK-8(H4) was the major respiratory quinone. The major cellular fatty acids were C16 : 0, C16 : 1 ω7, C16 : 1 ω5 and C17 : 1 ω6. A phylogenetic analysis based on the 16S rRNA gene sequence placed the strain in the class Deltaproteobacteria. The recognized bacterium most closely related to strain MSL86T was [Desulfobacterium] catecholicum DSM 3882T (sequence similarity 94.4 %), and the next most closely related recognized species were Desulfotalea psychrophila (94.2 % sequence similarity with the type strain) and Desulfotalea arctica (93.7 %). As the physiological and chemotaxonomic characteristics of MSL86T were distinctly different from those of any related species, a novel genus and species Desulfopila aestuarii gen. nov., sp. nov. are proposed to accommodate the strain. The type strain of Desulfopila aestuarii is MSL86T (=JCM 14042T=DSM 18488T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64600-0