1887

Abstract

A Gram-negative, non-spore-forming, yellow-pigmented bacterium, strain N4, was isolated from a nickel-complexed cyanide-degrading bioreactor and subjected to a polyphasic taxonomic study. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain N4 is affiliated to the genus of the family . The levels of 16S rRNA gene sequence similarity between strain N4 and the type strains of all known species were 93.2–95.8 %, suggesting that strain N4 represents a novel species within the genus . The strain contained iso-C and summed feature 4 as the major fatty acids and menaquinone MK-6 as the predominant respiratory quinone. The G+C content of the genomic DNA was 38.2 mol%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain N4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is N4 (=KCTC 12506=CCBAU 10201=DSM 17710).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64599-0
2007-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/1/141.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64599-0&mimeType=html&fmt=ahah

References

  1. de Beer H., Hugo C. J., Jooste P. J., Willems A., Vancanneyt M., Coenye T., Vandamme P. 2005; Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken-processing plant. Int J Syst Evol Microbiol 55:2149–2153 [CrossRef]
    [Google Scholar]
  2. de Beer H., Hugo C. J., Jooste P. J., Vancanneyt M., Coenye T., Vandamme P. 2006; Chryseobacterium piscium sp. nov., isolated from fish of the South Atlantic Ocean off South Africa. Int J Syst Evol Microbiol 56:1317–1322 [CrossRef]
    [Google Scholar]
  3. Fautz E., Reichenbach H. 1980; A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Gallego V., García M. T., Ventosa A. 2006; Chryseobacterium hispanicum sp. nov., isolated from the drinking water distribution system of Sevilla, Spain. Int J Syst Evol Microbiol 56:1589–1592 [CrossRef]
    [Google Scholar]
  6. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  7. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  8. Hugo C. J., Jooste P. J., Segers P., Vancanneyt M., Kersters L. 1999; A polyphasic taxonomic study of Chryseobacterium strains isolated from dairy sources. Syst Appl Microbiol 22:586–595 [CrossRef]
    [Google Scholar]
  9. Hugo C., Segers P., Hoste B., Vancanneyt M., Kersters K. 2003; Chryseobacterium joostei sp. nov., isolated from the dairy environment. Int J Syst Evol Microbiol 53:771–777 [CrossRef]
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol  3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  11. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H. J. 2003; Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53:93–97 [CrossRef]
    [Google Scholar]
  12. Kim K. K., Bae H. S., Schumann P., Lee S. T. 2005a; Chryseobacterium daecheongense sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 55:133–138 [CrossRef]
    [Google Scholar]
  13. Kim K. K., Kim M. K., Lim J. H., Park H. Y., Lee S. T. 2005b; Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. Int J Syst Evol Microbiol 55:1287–1293 [CrossRef]
    [Google Scholar]
  14. Komagata K., Suzuki K. I. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
    [Google Scholar]
  15. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  16. Li Y., Kawamura K., Fujiwara N., Naka T., Liu H., Huang X., Kobayashi K., Ezaki T. 2003; Chryseobacterium miricola sp. nov., a novel species isolated from condensation water of space station Mir. Syst Appl Microbiol 26:523–528 [CrossRef]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  18. Mudarris M., Austin B., Segers P., Vancanneyt M., Hoste B., Bernardet J. F. 1994; Flavobacterium scophthalmum sp. nov., a pathogen of turbot ( Scophthalmus maximus L.). Int J Syst Bacteriol 44:447–453 [CrossRef]
    [Google Scholar]
  19. Quan Z. X., Bae H. S., Baek J. H., Chen W. F., Im W. T., Lee S. T. 2005; Rhizobium daejeonense sp. nov. isolated from a cyanide treatment bioreactor. Int J Syst Evol Microbiol 55:2543–2549 [CrossRef]
    [Google Scholar]
  20. Quan Z. X., Rhee S. K., Bae J. W., Baek J. H., Park Y. H., Lee S. T. 2006; Bacterial community structure in activated sludge reactors treating free or metal-complexed cyanides. J Microbiol Biotechnol 16:232–249
    [Google Scholar]
  21. Rzhetsky A., Nei M. 1992; A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9:945–967
    [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  23. Sasser M. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids , MIDI. Technical Note 101: Newark, DE: MIDI;
    [Google Scholar]
  24. Shen F. O., Kämpfer P., Young C. C., Lai W. A., Arun A. B. 2005; Chryseobacterium taichungense sp. nov., isolated from contaminated soil. Int J Syst Evol Microbiol 55:1301–1304 [CrossRef]
    [Google Scholar]
  25. Shimomura K., Kaji S., Hiraishi A. 2005; Chryseobacterium shigense sp. nov., a yellow-pigmented aerobic bacterium isolated from a lactic acid beverage. Int J Syst Evol Microbiol 55:1903–1906 [CrossRef]
    [Google Scholar]
  26. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rDNA sequence analysis in the present species identification in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  27. Swofford D. L. 1993 paup Phylogenetic Analysis Using Parsimony, version 3.1.1 Champaign, IL: Illinois Natural History Survey;
    [Google Scholar]
  28. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  29. Vandamme P., Bernardet J. F., Segers P., Kersters K., Holmes B. 1994; New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen.nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44:827–831 [CrossRef]
    [Google Scholar]
  30. Weon H. Y., Kim B. Y., Yoo S. H., Kwon S. W., Cho Y. H., Go S. J., Stackebrandt E. 2006; Chryseobacterium wanjuense sp. nov., isolated from greenhouse soil in Korea. Int J Syst Evol Microbiol 56:1501–1504 [CrossRef]
    [Google Scholar]
  31. Yamaguchi S., Yokoe M. 2000; A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Appl Environ Microbiol 66:3337–3343 [CrossRef]
    [Google Scholar]
  32. Young C. C., Kämpfer P., Shen E. T., Lai W. A., Arun A. B. 2005; Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55:423–426 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64599-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64599-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error