1887

Abstract

Two strains belonging to the genus , HAW-EB2 and HAW-EB5, were isolated previously from marine sediment sampled from the Atlantic Ocean, near Halifax harbour in Canada, for their potential to degrade explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). In the present study, strains HAW-EB2 and HAW-EB5 were found to display high 16S rRNA gene sequence similarity (90–99.5 %) to species of , but their sequences were significantly different from each other and from species of (79–87.6 %). Furthermore, DNA–DNA hybridization showed that the genomic DNA of the two strains was only 22 % related and showed less than 41 % relatedness to closely related species of . In comparison to other species of , strains HAW-EB2 and HAW-EB5 were also unique in some phenotypic properties such as activities of -galactosidase and tyrosine arylamidase and the ability to metabolize certain organic acids and sugars. Both strains HAW-EB2 and HAW-EB5 utilize malate, valerate, peptone and yeast extract as sole carbon and energy sources. The major membrane fatty acids of the two strains were C, iso-C, C, C 7, C 7 and C 3 and their major quinones were Q-7, Q-8 and MK-7. On the basis of these results, strain HAW-EB2 (=NCIMB 14238 =CCUG 54553) is proposed as the type strain of sp. nov. and strain HAW-EB5 (=NCIMB 14239 =CCUG 54554) is proposed as the type strain of sp. nov.

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2007-09-01
2024-03-29
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