RT Journal Article SR Electronic(1) A1 Liu, Zhi-Pei A1 Wang, Bao-Jun A1 Dai, Xin A1 Liu, Xing-Yu A1 Liu, Shuang-JiangYR 2006 T1 Zhouia amylolytica gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from sediment of the South China Sea JF International Journal of Systematic and Evolutionary Microbiology, VO 56 IS 12 SP 2825 OP 2829 DO https://doi.org/10.1099/ijs.0.64587-0 PB Microbiology Society, SN 1466-5034, AB Three Gram-negative, non-spore-forming strains were isolated from sediment from the South China Sea, China, and their taxonomic positions were investigated using a polyphasic approach. Strains HN-171T, HN-172 and HN-181 grew optimally at 30 °C, in the presence of 4.5–5.0 NaCl % (w/v) and at pH 7.2–7.4. They contained MK-6 as the predominant respiratory quinone and contained iso-C15 : 1 G, iso-C15 : 0, summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c/t) and C15 : 0 as the major fatty acids. The DNA G+C content of strain HN-171T was 34.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that strain HN-171T, together with strains HN-172 and HN-181, formed a distinct evolutionary lineage within the family Flavobacteriaceae. The 16S rRNA gene sequences of strains HN-171T, HN-172 and HN-181 shared 99.8–100 % similarity with each other, and the sequence of strain HN-171T exhibited similarity values below 90.2 % with those of other members of the family Flavobacteriaceae. The closest relative of HN-171T was Coenonia anatina LMG 14382T (90.2 %). On the basis of their phenotypic and phylogenetic properties, the three isolates represent a novel genus and a novel species, for which the name Zhouia amylolytica gen. nov., sp. nov. is proposed. The type strain is HN-171T (=CGMCC 1.6114T=JCM 14016T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64587-0