A yellow-pigmented, Gram-negative, aerobic bacterial strain, designated 5YN10-9, was isolated from a wetland, Yongneup, of the Inje region, Korea. Cells were motile by means of one polar flagellum. Based on 16S rRNA gene sequence analyses, strain 5YN10-9 was shown to be related to the genera , , and , showing moderate sequence similarities of 90.2–93.3, 92.7, 90.9–93.3 and 92.4–92.6 % to members of these genera, respectively. Its distinct phylogenetic position and the low 16S rRNA gene sequence similarity values toward the closest related genera support the inclusion of this novel isolate in a new genus. The major isoprenoid quinone was ubiquinone 8 (Q-8). The predominant cellular fatty acids were C and summed feature 3 (comprising C 7 and/or iso-C 2-OH). The DNA G+C content was 59.3 mol%. The results of phenotypic, chemotaxonomic and phylogenetic analyses indicate that strain 5YN10-9 represents a novel species of a new genus within the family , class , for which the name gen. nov., sp. nov. is proposed. The type strain is 5YN10-9 (=KACC 11603=DSM 18521).


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  1. Achenbach, L. A., Michaelidou, U., Bruce, R. A., Fryman, J. & Coates, J. D.(2001).Dechloromonas agitata gen. nov., sp. nov. and Dechlorosoma suillum gen. nov., sp. nov., two novel environmentally dominant (per)chlorate-reducing bacteria and their phylogenetic position. Int J Syst Evol Microbiol 51, 527–533. [Google Scholar]
  2. Brune, A., Ludwig, W. & Schink, B.(2002).Propionivibrio limicola sp. nov., a fermentative bacterium specialized in the degradation of hydroaromatic compounds, reclassification of Propionibacter pelophilus as Propionivibrio pelophilus comb. nov. and amended description of the genus Propionivibrio. Int J Syst Evol Microbiol 52, 441–444. [Google Scholar]
  3. Cummings, D. E., Caccavo, F., Spring, S. & Rosenzweig, R. F.(1999).Ferribacterium limneticum, gen. nov., sp. nov., an Fe(III)-reducing microorganism isolated from mining-impacted freshwater lake sediments. Arch Microbiol 171, 183–188.[CrossRef] [Google Scholar]
  4. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  5. Garrity, G. M. & Holt, J. G.(2001). The road map to the Manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, pp. 119–166. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  6. Garrity, G. M., Bell, J. A. & Lilburn, T.(2005). Family I Rhodocyclaceae fam. nov. In Bergey'sManual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 887–922. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  7. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A.(1996).Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef] [Google Scholar]
  8. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  9. Kwon, S. W., Kim, J. S., Park, I. C., Yoon, S. H., Park, D. H., Lim, C. K. & Go, S. J.(2003).Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53, 21–27.[CrossRef] [Google Scholar]
  10. Maszenan, A. M., Seviour, R. J., Patel, B. K. C. & Schumann, P.(2002).Quadricoccus australiensis gen. nov., sp. nov., a β-proteobacterium from activated sludge biomass. Int J Syst Evol Microbiol 52, 223–228. [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  12. Reinhold-Hurek, B. & Hurek, T.(2000). Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato and description of three new genera and new species, Azovibrio restrictus gen. nov., sp. nov., Azospira oryzae gen. nov., sp. nov. and Azonexus fungiphilus gen. nov., sp. nov. Int J Syst Evol Microbiol 50, 649–659.[CrossRef] [Google Scholar]
  13. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  14. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  15. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  16. Song, B., Palleroni, N. J., Kerkhof, L. J. & Haggblom, M. M.(2001). Characterization of halobenzoate-degrading, denitrifying Azoarcus and Thauera isolates and description of Thauera chlorobenzoica sp. nov. Int J Syst Evol Microbiol 51, 589–602. [Google Scholar]
  17. Tarlera, S. & Denner, E. B. M.(2003).Sterolibacterium denitrificans gen. nov., sp. nov., a novel cholesterol-oxidizing, denitrifying member of the β-Proteobacteria. Int J Syst Evol Microbiol 53, 1085–1091.[CrossRef] [Google Scholar]
  18. Xie, C. H. & Yokota, A.(2006).Zoogloea oryzae sp. nov., a nitrogen-fixing bacterium isolated from rice paddy soil, and reclassification of strain ATCC 19623 as Crabtreella saccharophila gen. nov., sp. nov. Int J Syst Evol Microbiol 56, 619–624.[CrossRef] [Google Scholar]

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