RT Journal Article SR Electronic(1) A1 Lu, Shipeng A1 Ryu, Seung Hyun A1 Chung, Bok Sil A1 Chung, Young Ryun A1 Park, Woojun A1 Jeon, Che OkYR 2007 T1 Simplicispira limi sp. nov., isolated from activated sludge JF International Journal of Systematic and Evolutionary Microbiology, VO 57 IS 1 SP 31 OP 34 DO https://doi.org/10.1099/ijs.0.64566-0 PB Microbiology Society, SN 1466-5034, AB A Gram-negative, motile, rod-shaped bacterium, designated strain EMB325T, was isolated from activated sludge that performed enhanced biological phosphorus removal in a sequencing batch reactor. The predominant fatty acids of strain EMB325T were summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH), C16 : 0, C18 : 1 ω7c and C18 : 1 ω7c 11-methyl. The strain contained phosphatidylethanolamine and diphosphatidylglycerol as polar lipids. The G+C content of the genomic DNA was 63.3 mol%. The major quinone was Q-8. Phylogenetic analysis of 16S rRNA gene sequences showed that strain EMB325T formed a phyletic lineage with members of the genus Simplicispira and was most closely related to Simplicispira psychrophila LMG 5408T and Simplicispira metamorpha DSM 1837T with similarities of 98.1 and 97.9 %, respectively. Levels of DNA–DNA relatedness between strain EMB325T and S. psychrophila LMG 5408T and S. metamorpha DSM 1837T were 28 and 23 %, respectively. On the basis of chemotaxonomic data and molecular characteristics, strain EMB325T is considered to represent a novel species within the genus Simplicispira, for which the name Simplicispira limi sp. nov. is proposed. The type strain is EMB325T (=KCTC 12608T=DSM 17964T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64566-0