A novel psychrophilic, yellow-pigmented and obligate aerobic bacterium, strain 0499T, was isolated from the China No.1 glacier. Strain 0499T displayed the common phenotypic and chemotaxonomic features of the genus Flavobacterium, containing menaquinone-6 (MK-6) as the major quinone and C15 : 0, iso-C15 : 0, C17 : 1ω6c and summed feature 3 (C16 : 1ω7c/iso-C15 : 0 2-OH) as the major fatty acids. Optimal growth occurred at 21 °C. The genomic DNA G+C content was 36.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 0499T was related to members of the genus Flavobacterium, sharing the highest sequence similarities with Flavobacterium succinicans (97.9 %), Flavobacterium granuli (97.4 %) and Flavobacterium hydatis (97.2 %). On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, a novel species Flavobacterium glaciei is proposed with strain 0499T (=CGMCC 1.5380T=JCM 13953T) as the type strain.
AslamZ.,
ImW.-T.,
KimM. K.,
LeeS.-T.2005; Flavobacterium granuli sp. nov., isolated from granules used in a wastewater treatment plant. Int J Syst Evol Microbiol 55:747–751[CrossRef]
BergeyD. H.,
HarrisonF. C.,
BreedR. S.,
HammerB. W.,
HuntoonF. M.
(editors) 1923Genus II. Flavobacterium gen.nov. In Bergey's Manual of Determinative Bacteriology pp 1–442 Baltimore: Williams & Wilkins;
BernardetJ. F.,
SegersP.,
VancanneytM.,
BertheF.,
KerstersK.,
VandammeP.1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148[CrossRef]
CollinsM. D.1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp 267–287 Edited by
GoodfellowM.,
MinnikinD. E.
London: Academic Press;
DongX.-Z.,
CaiM.-Y.
(editors) 2001; Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria pp 370–398 Beijing: Science Press (in Chinese;
HornM. A.,
IhssenJ.,
MatthiesC.,
SchrammA.,
AckerG.,
DrakeH. L.2005; Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa
. Int J Syst Evol Microbiol 55:1255–1265[CrossRef]
HußV. A. R.,
FestlH.,
SchleiferK.-H.1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192[CrossRef]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[CrossRef]
McCammonS. A.,
BowmanJ. P.2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [ Flavobacterium ] salegens as Salegentibacter salegens gen. nov., comb. nov.. Int J Syst Evol Microbiol501055–1063[CrossRef]
ReichenbachH.1989; Order I. Cytophagales Leadbetter 1974. In Bergey's Manual of Systematic Bacteriology vol 3 pp 2011–2013 Edited by
StaleyJ. T.,
BryantM. P.,
PfennigN.,
HoltJ. G.
Baltimore: Williams & Wilkins;
SlyL. I.,
BlackallL. L.,
KraatP. C.,
Tian-ShenT.,
SangkhobolV.1986; The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5:139–156[CrossRef]