@article{mbs:/content/journal/ijsem/10.1099/ijs.0.64560-0, author = "Zhang, Li and Xu, Zhihong and Patel, Bharat K. C.", title = "Frondicola australicus gen. nov., sp. nov., isolated from decaying leaf litter from a pine forest", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2007", volume = "57", number = "6", pages = "1177-1182", doi = "https://doi.org/10.1099/ijs.0.64560-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64560-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen", abstract = "An aerobic bacterium, designated strain E1HC-02T, was isolated from the decaying leaf litter of a slash pine forest located in southeast Queensland, Australia. Cells of strain E1HC-02T were short irregular rods (0.5–1.0×0.2–0.4 μm) which stained Gram-positive and possessed a cell-wall ultrastructure which appeared to be made of protein subunits. The novel strain grew optimally in 1 % trypticase soy broth (TSB) at 25 °C and at a pH of 9.1. Strain E1HC-02T metabolized a range of carbohydrates, organic acids and amino acids. The G+C content of the DNA was 71±1 mol% as determined by the thermal denaturation method. 16S rRNA gene sequence analysis of strain E1HC-02T showed that it was a member of the family Microbacteriaceae, phylum Actinobacteria. The cell wall contained a type B2β peptidoglycan, the dominant cellular fatty acid was 18 : 1ω7c and the major hydroxy fatty acid was 2-OH 14 : 0. The major menaquinones were MK-8 (76 %) and MK-7 (24 %) and the glycolipids present were disphosphatidylglycerol, phosphatidylglycerol and three unidentified phospholipids. The chemotaxonomic properties of strain E1HC-02T were distinctly different to all of the 17 genera of the family Microbacteriaceae and hence strain E1HC-02T is designated as representing a novel species of a new genus, Frondicola australicus gen. nov., sp. nov. The type strain of the type species is E1HC-02T (=JCM 13598T=DSM 17894T).", }