1887

Abstract

Ten new -like strains were isolated from freshwater of the hard-water creek Westerhöfer Bach, northern Germany. These strains formed two phylogenetic groups: strains WB 1.1-56, WB 1.1-04, WB 1.1-14, WB 1.1-57 and WB 1.1-63; and strains WB 4.2-33, WB 4.1-86, WB 4.2-34, WB 4.2-32 and WB 4.2-78. Cells were Gram-negative, yellow-pigmented, chemoheterotrophic rods. Their major fatty acid profiles were similar, consisting of iso-C, iso-C 3-OH, iso-C 3-OH and summed feature 3 (C 7 and/or iso-C 2-OH). DNA G+C contents for strains WB 1.1-56 and WB 4.2-33 were 33.5 and 37.5 mol%, respectively. Phylogenetic analysis based on almost complete 16S rRNA gene sequences indicated that strain WB 1.1-56 was phylogenetically most closely related to KUC-1, and that strain WB 4.2-33 was related most closely to KUC-1 and DSM 1811. Levels of 16S rRNA gene sequence similarity between strains WB 1.1-56 and WB 4.2-33 and the type strains of recognized members of the genus were below 98 %. DNA–DNA hybridization experiments confirmed the separate genomic status of strains WB 1.1-56 and WB 4.2-33. Strains WB 1.1-56 and WB 4.2-33 and their respective relatives differed from phylogenetically related species based on several phenotypic characteristics. On the basis of their phenotypic and phylogenetic distinctiveness, the two groups of strains are considered to represent two novel species, for which the names sp. nov. (type strain WB 1.1-56=DSM 18293=CIP 109242) and sp. nov. (type strain WB 4.2-33=DSM 18292=CIP 109241) are proposed.

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2007-02-01
2019-10-20
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Fatty acid compositions of strains of and spp. nov. and some phylogenetically related reference type strains of the genus . [PDF](29 KB)

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Dendrogram showing relationships among the two novel strain clusters and phylogenetically neighbouring reference strains based on fatty acid compositions. [PDF](18 KB)

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