1887

Abstract

Four bacterial strains (E8, E49, I77 and N129) were isolated from the residual wash-water produced during the processing of Spanish-style green table olives. The isolates were subjected to a polyphasic taxonomic study using phenotypic, phylogenetic and genotypic methods. The bacteria were Gram-positive, spore-forming rods. Moreover, they were heterotrophs that were able to utilize cellobiose, glucose, mannose and rhamnose as carbon sources. The G+C content of their genomic DNA ranged from 30.7 to 33.4 mol%. The major cellular fatty acids found in strain E8 were iso-C, anteiso-C, iso-C and anteiso-C. DNA–DNA hybridization shows 76.2–88.3 % relatedness among the four strains. The 16S rRNA gene sequence of isolate E8 shows that it belongs to the genus , with the highest sequence similarity (99 %) to 123. However, phenotypic differences and DNA–DNA relatedness between strain E8 and ATCC 700626 of less than 47 % suggest the placement of these strains within a novel species of the genus . The name sp. nov. is proposed, with strain E8 (=LMG 23503=DSM 18098) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64550-0
2007-05-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/5/906.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64550-0&mimeType=html&fmt=ahah

References

  1. Arahal, D. R., Márquez, M. C., Volcani, B. E., Schleifer, K. H. & Ventosa, A. ( 1999; ). Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea. Int J Syst Bacteriol 49, 521–530.[CrossRef]
    [Google Scholar]
  2. Arahal, D. R., Márquez, M. C., Volcani, B. E., Schleifer, K. H. & Ventosa, A. ( 2000; ). Reclassification of Bacillus marismortui as Salibacillus marismortui comb. nov. Int J Syst Evol Microbiol 50, 1501–1503.[CrossRef]
    [Google Scholar]
  3. Bauer, A. W., Kirby, W. M. M., Sherris, J. C. & Turck, M. ( 1966; ). Antibiotic susceptibility by a standardized single disk method. Am J Clin Pathol 45, 493–496.
    [Google Scholar]
  4. Brenes, M. & de Castro, A. ( 1997; ). Transformation of oleuropein and its hydrolysis products during Spanish-style green olive processing. J Sci Food Agric 77, 353–358.
    [Google Scholar]
  5. Chenna, R., Sugawara, H., Koike, T., López, R., Gibson, T. J., Higgins, D. G. & Thomson, J. D. ( 2003; ). Multiple sequence alignment with the clustal series of programs. Nucleic Acids Res 31, 3497–3500.[CrossRef]
    [Google Scholar]
  6. Claus, D. & Berkeley, R. C. W. ( 1986; ). Genus Bacillus Cohn 1872, 174AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1105–1139. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  7. Ferragut, C. & Leclerc, H. ( 1976; ). Etude comparative des methodes de determination du Tm de l'ADN bacterien. Ann Microbiol 127, 223–235 (in French).
    [Google Scholar]
  8. Garabito, M. J., Arahal, D. R., Mellado, E., Márquez, M. C. & Ventosa, A. ( 1997; ). Bacillus salexigens sp. nov., a new moderately halophilic Bacillus species. Int J Syst Bacteriol 47, 735–741.[CrossRef]
    [Google Scholar]
  9. Heyndrickx, M., Lebbe, L., Kersters, K., Hoste, B., De Wachter, R., De Vos, P., Forsyth, G. & Logan, N. A. ( 1999; ). Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998 . Int J Syst Bacteriol 49, 1083–1090.[CrossRef]
    [Google Scholar]
  10. Heyrman, J., Logan, N. A., Busse, H.-J., Balcaen, A., Lebbe, L., Rodríguez-Díaz, M., Swings, J. & De Vos, P. ( 2003; ). Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int J Syst Evol Microbiol 53, 501–503.[CrossRef]
    [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  12. Lee, J.-S., Lim, J.-M., Lee, K. C., Lee, J.-C., Park, Y.-H. & Kim, C.-J. ( 2006; ). Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions. Int J Syst Evol Microbiol 56, 251–257.[CrossRef]
    [Google Scholar]
  13. Lind, E. & Ursing, J. ( 1986; ). Clinical strains of Enterobacter agglomerans (synonyms: Erwinia herbicola, Erwinia milletiae) identified by DNA-DNA-hybridization. Acta Pathol Microbiol Immunol Scand [B] 94, 205–213.
    [Google Scholar]
  14. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  15. Owen, R. & Hill, L. R. ( 1979; ). The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists, 2nd edn, pp. 227–296. Edited by F. A. Skinner & D. W. Lovelock. London: Academic Press.
  16. Rodríguez-Valera, F., Ruíz-Berraquero, F. & Ramos-Cormenzana, A. ( 1981; ). Characteristics of the heterotrophic population in hypersaline environments of different salt concentration. Microb Ecol 7, 235–243.[CrossRef]
    [Google Scholar]
  17. Romero Barranco, C., Brenes Balbuena, M., García García, P. & Garrido Fernández, A. ( 2001; ). Management of spent brines or osmotic solutions. J Food Eng 49, 237–246.[CrossRef]
    [Google Scholar]
  18. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Bacteriology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Philips. Washington, DC: American Society for Microbiology.
  19. Ventosa, A., Quesada, E., Rodríguez-Valera, F., Ruíz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  20. Wainø, M., Tindall, B. J., Schumann, P. & Ingvorsen, K. (1999; ). Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49, 821–831.[CrossRef]
    [Google Scholar]
  21. Yoon, J.-H., Oh, T.-K. & Park, Y.-H. ( 2004; ). Transfer of Bacillus halodenitrificans Denariaz et al. 1989 to the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov. Int J Syst Evol Microbiol 54, 2163–2167.[CrossRef]
    [Google Scholar]
  22. Yoon, J.-H., Kang, S.-J., Lee, S.-Y., Lee, M.-H. & Oh, T.-K. ( 2005; ). Virgibacillus dokdonensis sp. nov., isolated from a Korean island, Dokdo, located at the edge of the East Sea in Korea. Int J Syst Evol Microbiol 55, 1833–1837.[CrossRef]
    [Google Scholar]
  23. Ziemke, F., Höfle, M. G., Lalucat, J. & Rosselló-Mora, R. ( 1998; ). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48, 179–186.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64550-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64550-0
Loading

Data & Media loading...

vol. , part 5, pp. 906 - 910

Phylogenetic tree showing relationships among strain E 8 , species and other related taxa, constructed using the maximum-parsimony algorithm and based on 16S rRNA gene sequences. [PDF](19 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error