1887

Abstract

Four bacterial strains (E8, E49, I77 and N129) were isolated from the residual wash-water produced during the processing of Spanish-style green table olives. The isolates were subjected to a polyphasic taxonomic study using phenotypic, phylogenetic and genotypic methods. The bacteria were Gram-positive, spore-forming rods. Moreover, they were heterotrophs that were able to utilize cellobiose, glucose, mannose and rhamnose as carbon sources. The G+C content of their genomic DNA ranged from 30.7 to 33.4 mol%. The major cellular fatty acids found in strain E8 were iso-C, anteiso-C, iso-C and anteiso-C. DNA–DNA hybridization shows 76.2–88.3 % relatedness among the four strains. The 16S rRNA gene sequence of isolate E8 shows that it belongs to the genus , with the highest sequence similarity (99 %) to 123. However, phenotypic differences and DNA–DNA relatedness between strain E8 and ATCC 700626 of less than 47 % suggest the placement of these strains within a novel species of the genus . The name sp. nov. is proposed, with strain E8 (=LMG 23503=DSM 18098) as the type strain.

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2007-05-01
2024-03-29
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