1887

Abstract

A Gram-positive, rod-shaped, spore-forming and moderately halophilic bacterium (strain 8-1) was isolated from a sediment sample of a neutral salt lake in Xin-Jiang, China. The strain grew optimally at 30 °C, pH 7.0–7.5 and 8–12 % (w/v) NaCl. The major cellular fatty acids were anteiso-C, iso-C and anteiso-C. The predominant isoprenoid quinone was MK-7 and the cell-wall peptidoglycan contained -diaminopimelic acid. Phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids were found to be the major polar lipid components. The genomic DNA G+C content was 43.4 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain 8-1 was closely related to KCTC 3911 (98.0 % sequence similarity) and other recognized species within the genus (94.5–95.9 %). The level of DNA–DNA relatedness between strain 8-1 and KCTC 3911 was 40 %. Based on the phenotypic and genotypic data presented, strain 8-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 8-1 (=CGMCC 1.3702=DSM 18342).

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2007-03-01
2020-01-29
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References

  1. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  2. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  3. Duckworth, A. W., Grant, W. D., Jones, B. E. & Steenbergen, R. V. ( 1996; ). Phylogenetic diversity of soda lake alkaliphiles. FEMS Microbiol Ecol 19, 181–191.[CrossRef]
    [Google Scholar]
  4. Gerhardt, P., Murray, R. G. E., Costilow, R. N., Nester, E. W., Woods, W. A., Krieg, N. R. & Philips, G. B. (editors) ( 1981; ). Manual of Methods for General Bacteriology. Washington, DC: American Society for Microbiology.
  5. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  6. Heyndrickx, M., Lebbe, L., Kersters, K., De Vos, P., Forsyth, G. & Logan, N. A. ( 1998; ). Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. Int J Syst Bacteriol 48, 99–106.[CrossRef]
    [Google Scholar]
  7. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  8. Jeon, C. O., Lim, J.-M., Lee, J. C., Lee, G. S., Lee, J.-M., Xu, L.-H., Jiang, C.-L. & Kim, C.-J. ( 2005; ). Lentibacillus salarius sp. nov., isolated from saline sediment in China, and emended description of the genus Lentibacillus. Int J Syst Evol Microbiol 55, 1339–1343.[CrossRef]
    [Google Scholar]
  9. Kodaka, H., Armfield, A. Y., Lombard, G. L. & Dowell, V. R., Jr ( 1982; ). Practical procedure for demonstrating bacterial flagella. J Clin Microbiol 16, 948–952.
    [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Kushner, D. J. ( 1978; ). Life in high salt and solute concentrations: halophilic bacteria. In Microbial Life in Extreme Environments, pp. 317–368. Edited by D. J. Kushner. London: Academic Press.
  12. Lim, J.-M., Jeon, C. O., Song, S.-M., Lee, J.-C., Ju, Y. J., Xu, L.-H., Jiang, C.-L. & Kim, C.-J. ( 2005; ). Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China. Int J Syst Evol Microbiol 55, 1805–1809.[CrossRef]
    [Google Scholar]
  13. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4, 109–118.
    [Google Scholar]
  14. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  15. Namwong, S., Tanasupawat, S., Smitinont, T., Visessanguan, W., Kudo, T. & Itoh, T. ( 2005; ). Isolation of Lentibacillus salicampi strains and Lentibacillus juripiscarius sp. nov. from fish sauce in Thailand. Int J Syst Evol Microbiol 55, 315–320.[CrossRef]
    [Google Scholar]
  16. Ren, P. G. & Zhou, P. J. ( 2005a; ). Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China. Int J Syst Evol Microbiol 55, 95–99.[CrossRef]
    [Google Scholar]
  17. Ren, P. G. & Zhou, P. J. ( 2005b; ). Salinibacillus aidingensis gen. nov., sp. nov. and Salinibacillus kushneri sp. nov., moderately halophilic bacteria isolated from a neutral saline lake in Xin-Jiang, China. Int J Syst Evol Microbiol 55, 949–953.[CrossRef]
    [Google Scholar]
  18. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  19. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  20. Schlesner, H., Lawson, P. A., Collins, M. D., Weiss, N., Wehmeyer, U., Völker, H. & Thomm, M. ( 2001; ). Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn–d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51, 425–431.
    [Google Scholar]
  21. Smibert, R. M. & Krieg, N. R. ( 1981; ). Phenotypic characterization. In Manual of Methods for General Microbiology, pp. 611–654. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Woods, N. R. Krieg & G. B. Philips. Washington, DC: American Society for Microbiology.
  22. Spring, S., Ludwig, W., Marquez, M. C., Ventosa, A. & Schleifer, K. H. ( 1996; ). Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov., and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46, 492–496.[CrossRef]
    [Google Scholar]
  23. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  24. Tanasupawat, S., Pakdeeto, A., Namwong, S., Thawai, C., Kudo, T. & Itoh, T. ( 2006; ). Lentibacillus halophilus sp. nov., from fish sauce in Thailand. Int J Syst Evol Microbiol 56, 1859–1863.[CrossRef]
    [Google Scholar]
  25. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  26. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  27. Wainø, M., Tindall, B. J., Schumann, P. & Ingvorsen, K. ( 1999; ). Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49, 821–831.[CrossRef]
    [Google Scholar]
  28. Xin, H., Itoh, T., Zhou, P., Suzuki, K. & Nakase, T. ( 2001; ). Natronobacterium nitratireducens sp. nov., a haloalkaliphilic archaeon isolated from a soda lake in China. Int J Syst Evol Microbiol 51, 1825–1829.[CrossRef]
    [Google Scholar]
  29. Yoon, J.-H., Weiss, N., Lee, K.-C., Lee, I.-S., Kang, K. H. & Park, Y.-H. ( 2001; ). Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with l-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen. nov., comb. nov. Int J Syst Evol Microbiol 51, 2087–2093.[CrossRef]
    [Google Scholar]
  30. Yoon, J.-H., Kang, K. H. & Park, Y.-H. ( 2002; ). Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. Int J Syst Evol Microbiol 52, 2043–2048.[CrossRef]
    [Google Scholar]
  31. Zhu, F., Wang, S. & Zhou, P. ( 2003; ). Flavobacterium xinjiangense sp. nov. and Flavobacterium omnivorum sp. nov., novel psychrophiles from the China No. 1 glacier. Int J Syst Evol Microbiol 53, 853–857.[CrossRef]
    [Google Scholar]
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