1887

Abstract

Two novel anamorphic yeast strains (S-15L and 3-C1) were isolated from the inflorescences of plants collected in two different towns in Rajasthan State, India. Sequencing of the D1/D2 domains of the large-subunit (LSU) rDNA and the internal transcribed spacer (ITS) regions suggested they are strains of the same species. Phenotypic characteristics such as the absence of fermentation, the absence of sexual structures and ballistoconidia, the assimilation of -inositol and -glucuronate, and positive Diazonium blue B and urease reactions indicated that these strains belong to the genus . The novel strains differed from in six physiological tests and differed from other related species in more than six tests. A phylogenetic analysis of the sequences of the D1/D2 domains of the LSU rDNA and the ITS regions placed these strains in the clade within the order Tremellales, with as their closest described relative. The novel strains showed 1.6 and 7.5 % divergence in the D1/D2 domain of the LSU rDNA and ITS regions, respectively, with respect to . The divergence from other species was more than 3 % for the D1/D2 domain and more than 9 % for the ITS region. On the basis of the phenotypic and molecular data, strains S-15L and 3-C1 represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is S-15L (=MTCC 7075=CBS 10406).

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2007-02-01
2020-01-23
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vol. , part 2, pp. 414 - 418

Neighbour-joining tree for strains S-15 and 3-C1 and related strains, based on sequences of the D1/D2 domain of the LSU rDNA. [PDF](15 KB)



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