1887

Abstract

A psychrophilic bacterium, designated strain HJ039, was isolated from a marine sponge collected in the East Sea of Korea (also known as the Sea of Japan). Cells were Gram-negative, motile and rod-shaped (1.8–3.54 μm×0.27–0.73 μm). Growth was observed between 5 and 26 °C (optimum 15 °C), at pH 5.0–8.5 (optimum pH 6.0–6.5) and in the presence of 0–6.0 % NaCl (optimum 2.0 %). The 16S rRNA gene sequence of strain HJ039 showed high levels of similarity (93.7–95.4 %) with members of the genus , especially with TF-27 (95.2 %), S12 (94.9 %), LMG 26268 (94.6 %), P010 (94.6 %), ATCC 51192 (94.5 %) and c931 (94.5 %). However, phylogenetic analysis revealed that strain HJ039 shared a phyletic line with and . The major respiratory quinone was Q-8. The DNA G+C content was 52.8 mol%. The major fatty acids were i-13 : 0 (8.5 %), 15 : 0 (4.2 %), i-15 : 0 (23.2 %), i-15 : 1 (7.9 %), 16 : 0 (8.7 %), 16 : 17 (21.0 %) and 17 : 18 (6.4 %). From this polyphasic taxonomic evidence, strain HJ039 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HJ039 (=KCCM 42304=JCM 13830).

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2006-12-01
2020-01-18
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References

  1. Aguirre, A. A., Balazas, G. H., Zimmerman, B. & Spraker, T. R. ( 1994; ). Evaluation of Hawaiian green turtles (Cheledonia mydas) for potential pathogens associated with fibropapillomas. J Wild Dis 30, 8–15.[CrossRef]
    [Google Scholar]
  2. Anzai, Y., Kim, H., Park, J. Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  3. Bowman, J. P. ( 2005; ). Genus XIII. Shewanella MacDonell and Colwell 1986 , 355VP (Effective publication: MacDonell and Colwell 1985 , 180). In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 480–491. Edited by D. J. Brenner, N. R. Crieg, J. T. Staley & G. M. Garrity. New York: Springer.
  4. Bozal, N., Montes, M. J., Tudela, E., Jiménez, F. & Guinea, J. ( 2002; ). Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol 52, 195–205.
    [Google Scholar]
  5. Brink, A. J., van Straten, A. & van Rensburg, A. J. ( 1995; ). Shewanella (Pseudomonas) putrefaciens bacteremia. Clin Infect Dis 20, 1327–1332.[CrossRef]
    [Google Scholar]
  6. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in bacterial classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  7. Gauthier, G., Gauthier, M. & Christen, R. ( 1995; ). Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int J Syst Bacteriol 45, 755–761.[CrossRef]
    [Google Scholar]
  8. Ivanova, E. P., Sawabe, T., Gorshkova, N. M., Svetashev, V. I., Mikhailov, V. V., Nicolau, D. V. & Christen, R. ( 2001; ). Shewanella japonica sp. nov. Int J Syst Evol Microbiol 51, 1027–1033.[CrossRef]
    [Google Scholar]
  9. Jorgensen, B. R. & Huß, H. H. ( 1989; ). Growth and activity of Shewanella putrefaciens isolated from spoiling fish. Int J Food Microbiol 9, 51–62.[CrossRef]
    [Google Scholar]
  10. Kostka, J. E., Stucki, J. W., Nealson, K. H. & Wu, J. ( 1996; ). Reduction of structural Fe(III) in smectite by a pure culture of Shewanella putrefaciens strain MR-1. Clays Clay Miner 44, 522–529.[CrossRef]
    [Google Scholar]
  11. Kwon, K. K., Lee, H.-S., Yang, S. H. & Kim, S.-J. ( 2005; ). Kordiimonas gwangyangensis gen. nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (Kordiimonadales ord. nov.) in the ‘Alphaproteobacteria’. Int J Syst Evol Microbiol 55, 2033–2037.[CrossRef]
    [Google Scholar]
  12. Lovley, D. R. & Phillips, E. J. P. ( 1988; ). Novel mode of microbial energy metabolism: organic carbon oxidation coupled to dissimilatory reduction of iron or manganese. Appl Environ Microbiol 54, 1472–1480.
    [Google Scholar]
  13. MacDonell, M. T. & Colwell, R. R. ( 1985; ). Phylogeny of the Vibrionaceae, and recommendation for two new genera, Listonella and Shewanella. Syst Appl Microbiol 6, 171–182.[CrossRef]
    [Google Scholar]
  14. Myers, C. R. & Nealson, K. H. ( 1988; ). Manganese reduction bacteria and growth with manganese oxide as the sole electron acceptor. Science 240, 1319–1321.[CrossRef]
    [Google Scholar]
  15. Nealson, K. H., Myers, C. R. & Wimpee, B. ( 1991; ). Isolation and identification of manganese reducing bacteria, and estimates of microbial manganese reducing potential in the Black Sea. Deep Sea Res 38, S907–S920.[CrossRef]
    [Google Scholar]
  16. Nozue, H., Hayashi, T., Hashimoto, Y., Ezaki, T., Hamasaki, K., Ohwada, K. & Terawaki, Y. ( 1992; ). Isolation and characterization of Shewanella algae from human clinical specimens and emendation of the description of S. algae Simidu et al., 1990, 335. Int J Syst Bacteriol 42, 628–634.[CrossRef]
    [Google Scholar]
  17. Perry, K. A., Kostka, J. E., Luther, G. W., III & Nealson, K. H. ( 1993; ). Mediation of sulfur speciation by a Black Sea facultative anaerobe. Science 259, 801–803.[CrossRef]
    [Google Scholar]
  18. Petrovskis, E. A., Vogel, T. M. & Adriaens, P. ( 1994; ). Effects of electron acceptors and donors on transformation of tetrachloromethane by Shewanella putrefaciens MR-1. FEMS Microbiol Lett 121, 357–364.[CrossRef]
    [Google Scholar]
  19. Satomi, M., Vogel, B. F., Gram, L. & Venkateswaran, K. ( 2006; ). Shewanella hafniensis sp. nov. and Shewanella morhuae sp. nov., isolated from marine fish of the Baltic Sea. Int J Syst Evol Microbiol 56, 243–249.[CrossRef]
    [Google Scholar]
  20. Semple, K. M. & Westlake, D. W. S. ( 1987; ). Characterization of iron reducing Alteromonas putrefaciens strains from oil field fluids. Can J Microbiol 35, 925–931.
    [Google Scholar]
  21. Sohn, J. H., Kwon, K. K., Kang, J.-H., Jung, H.-B. & Kim, S.-J. ( 2004; ). Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol 54, 1483–1487.[CrossRef]
    [Google Scholar]
  22. Stackebrandt, E. & Liesack, W. ( 1993; ). Nucleic acids and classification. In Handbook of New Bacterial Systematics, pp. 158–160. Edited by M. Goodfellow & A. G. O'Donnell. London: Academic Press.
  23. Venkateswaran, K., Moser, D. P., Dollhopf, M. E. & 10 other authors ( 1999; ). Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol 49, 705–724.[CrossRef]
    [Google Scholar]
  24. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
  25. Xu, M., Guo, J., Cen, Y., Zhong, X., Cao, W. & Sun, G. ( 2005; ). Shewanella decolorationis sp. nov., a dye-decolorizing bacterium isolated from activated sludge of a waste-water treatment plant. Int J Syst Evol Microbiol 55, 363–368.[CrossRef]
    [Google Scholar]
  26. Yoon, J.-H., Kang, K. H., Oh, T.-K. & Park, Y.-H. ( 2004; ). Shewanella gaetbuli sp. nov., a slight halophile isolated from a tidal flat in Korea. Int J Syst Evol Microbiol 54, 487–491.[CrossRef]
    [Google Scholar]
  27. Zhao, J.-S., Manno, D., Beaulieu, C., Paquet, L. & Hawari, J. ( 2005; ). Shewanella sediminis sp. nov., a novel Na+-requiring and hexahydro-1,3,5-trinitro-1,3,5-triazine-degrading bacterium from marine sediment. Int J Syst Evol Microbiol 55, 1511–1520.[CrossRef]
    [Google Scholar]
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vol. , part 12, pp. 2879-2882

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