1887

Abstract

Two strains (S-34 and S-35) of a novel ascomycetous yeast species belonging to the genus were isolated from soil from a mixed deciduous forest in Amphoe Wang Nam Khiao, Nakhon Ratchasima Province, Thailand. The D1/D2 domains of the large-subunit rDNA sequences of the two strains were identical and also indicated a close relationship with respect to , , , and . The most closely related species, , has 14 nucleotide substitutions and three gaps in 566 nt. The phenotypic characteristics of the two strains were typical of those of members of the genus . These characteristics include the formation of a single globose ascospore in an unconjugated and persistent ascus, multilateral budding, the absence of arthrospores and ballistospores, the fermentation of glucose, the inability to assimilate nitrate, negative diazonium blue B and urease reactions, and the presence of ubiquinone Q-6. The novel strains can be distinguished from on the basis of a number of phenotypic characteristics and represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is S-34 (=CBS 10361=NBRC 101968=BCC 21230).

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2007-02-01
2024-04-19
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References

  1. Altschul S. F., Gish W., Miller W., Meyers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  3. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  4. Kuraishi H., Katayama-Fujimura Y., Sugiyama J., Yokoyama T. 1985; Ubiquinone systems in fungi I. Distribution of ubiquinones in the major families of ascomycetes, basidiomycetes, and deuteromycetes, and their taxonomic implications. Trans Mycol Soc Jpn 26:383–395
    [Google Scholar]
  5. Kurtzman C. P. 2003; Phylogenetic circumscription of Saccharomyces , Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea ,Nakaseomyces , Naumovia , Vanderwaltozyma and Zygotorulaspora . FEMS Yeast Res 4:233–245 [CrossRef]
    [Google Scholar]
  6. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large-subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371 [CrossRef]
    [Google Scholar]
  7. Kurtzman C. P., Robnett C. J. 2003; Phylogenetic relationships among yeasts of the ‘ Saccharomyces complex’ determined from multigene sequence analysis. FEMS Yeast Res 3:417–432 [CrossRef]
    [Google Scholar]
  8. Kurtzman C. P., Robnett C. J., Ward J. M., Brayton C., Gorelick P., Walsh T. J. 2005; Multigene phylogenetic analysis of pathogenic Candida species in the Kazachstania ( Arxiozyma ) telluris complex and description of their ascosporic states as Kazachstania bovina sp.nov., K.heterogenica sp. nov., K. pintolopsii sp. nov., and K. slooffiae sp. nov.. J Clin Microbiol 43:101–111 [CrossRef]
    [Google Scholar]
  9. Lachance M. A., Bowles J. M., Starmer W. T., Baker S. F. 1999; Kodamaea kadaduensis and Candida tolerans , two new ascomycetous yeast species from Australian Hibiscus flowers. Can J Microbiol 45:172–177 [CrossRef]
    [Google Scholar]
  10. Lu H.-Z., Cai Y., Wu Z.-W., Jia J.-H., Bai F.-Y. 2004; Kazachstania aerobia sp. nov., an ascomycetous yeast species from aerobically deteriorating corn silage. Int J Syst Evol Microbiol 54:2431–2435 [CrossRef]
    [Google Scholar]
  11. Nakase T., Suzuki M. 1986; Bullera megalospora , a new species of yeast forming larger ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan. J Gen Appl Microbiol 32:225–240 [CrossRef]
    [Google Scholar]
  12. O'Donnell K. 1993; Fusarium and its near relatives. In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics pp  225–233 Edited by Reynolds D. R., Taylor J. W. Wallingford, UK: CAB International;
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Sjamsuridzal W., Tajiri Y., Nishida H., Thuan T. B., Kawasaki H., Hirata A., Yokota A., Sugiyama J. 1997; Evolutionary relationships of members of the genera Taphrina , Protomyces , Schizosaccharomyces and related taxa within the Archiascomycetes: integrated analysis of genotypic characteristics. Mycoscience 38:267–280 [CrossRef]
    [Google Scholar]
  15. Thompson J. D., Higgins D. G., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. J. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  16. Wu Z.-W., Bai F.-Y. 2005; Kazachstania aquatica sp. nov. and Kazachstania solicola sp. nov., novel ascomycetous yeast species. Int J Syst Evol Microbiol 55:2219–2224 [CrossRef]
    [Google Scholar]
  17. Yamada Y., Kondo K. 1973; Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast-like genera Sporobolomyces and Rhodosporidium . J Gen Appl Microbiol 19:59–77 [CrossRef]
    [Google Scholar]
  18. Yamada Y., Kawasaki H., Nagatsuka Y., Mikata K., Seki T. 1999; The phylogeny of the catophilic yeasts based on the 18S ribosomal RNA gene sequences: the proposals of Phaffomyces antillensis and Starmera caribaea , new combinations. Biosci Biotechnol Biochem 63:827–832 [CrossRef]
    [Google Scholar]
  19. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study , 4th edn. pp  77–100 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
  20. Zubkova R. D. 1971; Genus novum Saccharomycetacearum e Kazachstania . Bot Mater Gerb Inst Bot Akad Nauk Kazakh SSR 753–56 (in Russian)
    [Google Scholar]
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