1887

Abstract

Two novel strains, SL014B61A and SL014B11A, were isolated from an oil-polluted saline soil from Gudao in the coastal Shengli Oilfield, eastern China. Cells of strains SL014B61A and SL014B11A were motile, Gram-negative and rod-shaped. Growth occurred at NaCl concentrations of between 0 and 15 % and at temperatures of between 10 and 45 °C. Strain SL014B61A had Q9 as the major respiratory quinone and C16 : 0 (21.2 %), C18 : 19 (20.3 %), C16 : 17 (7.3 %) and C16 : 19 (6.4 %) as predominant fatty acids. The G+C content of the DNA was 57.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SL014B61A belonged to the genus in the class . Strain SL014B61A showed the highest 16S rRNA gene sequence similarity with (97.9 %) and showed 97.8 % sequence similarity to . DNA–DNA relatedness to the reference strains and was 35.5 % and 33.8 %, respectively. On the basis of these data, it is proposed that strains SL014B61A and SL014B11A represent a novel species, sp. nov. The type strain is strain SL014B61A (=DSM 18066=LMG 23509=CGMCC 1.6294).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64522-0
2007-02-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/2/250.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64522-0&mimeType=html&fmt=ahah

References

  1. Cho, J.-C. & Giovannoni, S. J. ( 2003; ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria. Int J Syst Evol Microbiol 53, 1031–1036.[CrossRef]
    [Google Scholar]
  2. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 2004; ). phylip (phylogeny inference package), version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  4. Gauthier, M. J., Lafay, B., Christen, R., Fernandez, L., Acquaviva, M., Bonin, P. & Bertrand, J.-C. ( 1992; ). Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon degrading marine bacterium. Int J Syst Bacteriol 42, 568–576.[CrossRef]
    [Google Scholar]
  5. Gorshkova, N. M., Ivanova, E. P., Sergeev, A. F., Zhukova, N. V., Alexeeva, Y., Wright, J. P., Nicolau, D. V., Mikhailov, V. V. & Christen, R. ( 2003; ). Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan. Int J Syst Evol Microbiol 53, 2073–2078.[CrossRef]
    [Google Scholar]
  6. Green, D. H., Bowman, J. P., Smith, E. A., Gutierrez, T. & Bolch, C. J. S. ( 2006; ). Marinobacter algicola sp. nov., isolated from laboratory cultures of paralytic shellfish toxin-producing dinoflagellates. Int J Syst Evol Microbiol 56, 523–527.[CrossRef]
    [Google Scholar]
  7. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  8. Johnson, J. L. ( 1994; ). Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology, pp. 655–682. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  9. Kim, B.-Y., Weon, H.-Y., Yoo, S.-H., Kim, J.-S., Kwon, S. W., Stackebrandt, E. & Go, S.-J. ( 2006; ). Marinobacter koreensis sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 56, 2653–2656.[CrossRef]
    [Google Scholar]
  10. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  11. Liebgott, P.-P., Casalot, L., Paillard, S., Lorquin, J. & Labat, M. ( 2006; ). Marinobacter vinifirmus sp. nov., a moderately halophilic bacterium isolated from a wine-barrel-decalcification wastewater. Int J Syst Evol Microbiol 56, 2511–2516.[CrossRef]
    [Google Scholar]
  12. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  13. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  14. Márquez, M. C. & Ventosa, A. ( 2005; ). Marinobacter hydrocarbonoclasticus Gauthier et al. 1992 and Marinobacter aquaeolei Nguyen et al. 1999 are heterotypic synonyms. Int J Syst Evol Microbiol 55, 1349–1351.[CrossRef]
    [Google Scholar]
  15. Martín, S., Márquez, M. C., Sánchez-Porro, C., Mellado, E., Arahal, D. R. & Ventosa, A. ( 2003; ). Marinobacter lipolyticus sp. nov., a novel moderate halophile with lipolytic activity. Int J Syst Evol Microbiol 53, 1383–1387.[CrossRef]
    [Google Scholar]
  16. Nguyen, B. H., Denner, E. B. M., Ha, D. T. C., Wanner, G. & Stan-Lotter, H. ( 1999; ). Marinobacter aquaeolei sp. nov., a halophilic bacterium isolated from a Vietnamese oil-producing well. Int J Syst Bacteriol 49, 367–375.[CrossRef]
    [Google Scholar]
  17. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef]
    [Google Scholar]
  18. Romanenko, L. A., Schumann, P., Rohde, M., Zhukova, N. V., Mikhailov, V. V. & Stackebrandt, E. ( 2005; ). Marinobacter bryozoorum sp. nov. and Marinobacter sediminum sp. nov., novel bacteria from the marine environment. Int J Syst Evol Microbiol 55, 143–148.[CrossRef]
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Shieh, W. Y., Jean, W. D., Lin, Y. T. & Tseng, M. ( 2003; ). Marinobacter lutaoensis sp. nov., a thermotolerant marine bacterium isolated from a coastal hot spring in Lutao, Taiwan. Can J Microbiol 49, 244–252.[CrossRef]
    [Google Scholar]
  21. Shivaji, S., Gupta, P., Chaturvedi, P., Suresh, K. & Delille, D. ( 2005; ). Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 55, 1453–1456.[CrossRef]
    [Google Scholar]
  22. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Kreig. Washington, DC: American Society for Microbiology.
  23. Spröer, C., Lang, E., Hobeck, P., Burghardt, J., Stackebrandt, E. & Tindall, B. J. ( 1998; ). Transfer of Pseudomonas nautica to Marinobacter hydrocarbonoclasticus. Int J Syst Bacteriol 48, 1445–1448.[CrossRef]
    [Google Scholar]
  24. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  25. Tindall, B. J. ( 1990; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  26. Yoon, J.-H., Shin, D.-Y., Kim, I.-G., Kang, K. H. & Park, Y.-H. ( 2003; ). Marinobacter litoralis sp. nov., a moderately halophilic bacterium isolated from sea water from the East Sea in Korea. Int J Syst Evol Microbiol 53, 563–568.[CrossRef]
    [Google Scholar]
  27. Yoon, J.-H., Yeo, S. H., Kim, I.-G. & Oh, T. K. ( 2004; ). Marinobacter flavimaris sp. nov. and Marinobacter daepoensis sp. nov., slightly halophilic organisms isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54, 1799–1803.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64522-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64522-0
Loading

Data & Media loading...

Supplements

vol. , part 2, pp. 250 - 254

Electron micrographs of cells of strain SL014B61A . [PDF](224 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error