1887

Abstract

Five novel endospore-forming, nitrogen-fixing bacterial strains were isolated from the rhizosphere soils of plants of the species , and . A phylogenetic analysis based on 16S rRNA gene sequences revealed that the five strains formed a distinct cluster within the genus . These novel strains showed the highest levels (96.2–98.2 %) of 16S rRNA gene sequence similarity with . However, the DNA–DNA relatedness between these novel strains and was 12.9–29.5 %. The DNA G+C contents of the five strains were found to be 51.9–52.9 mol%. Phenotypic analyses showed that a significant feature of the novel strains (differentiating them from and other species) is that all of them were unable to produce acid and gas from various carbohydrates such as glucose, sucrose, lactose and fructose. Anteiso-branched C was the major fatty acid present in the novel type strain. On the basis of these data, the five novel strains represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T27 (=CCBAU 10202=DSM 17841).

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2007-01-01
2020-01-27
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