sp. nov., a thermophilic, moderately halophilic, fermentative bacterium isolated from an offshore oil well in Congo Free

Abstract

A novel thermophilic, moderately halophilic, rod-shaped bacterium, strain MET-B, with a sheath-like outer structure (toga) was isolated from an offshore oil-producing well in Congo, West Africa. Strain MET-B was a Gram-negative bacterium with the ability to reduce elemental sulfur, but not sulfate, thiosulfate or sulfite into sulfide. The optimum growth conditions were 60 °C, pH 6.7–7.2 and 4–6 % NaCl. The DNA G+C content was 34.6 mol%. Strain MET-B was phylogenetically related to members of the genus ; , and were the closest relatives, with type strains exhibiting more than 99 % identity in an analysis of small-subunit rRNA gene sequences. The values for DNA–DNA relatedness between the type strains of these three species and strain MET-B were less than 42 %. As MET-B was found to be genetically and physiologically different from other species of the genus , this strain is proposed as representing a novel species, for which the name sp. nov. is proposed. The type strain is MET-B (=DSM 16923=CCUG 50214).

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2007-01-01
2024-03-28
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References

  1. Balch W. E., Fox G. E., Magrum R. J., Wolfe R. S. 1979; Methanogens: reevaluation of a unique biological group. Microbiol Rev 43:260–296
    [Google Scholar]
  2. Benson D., Boguski M. S., Lipman D. J., Ostell J., Ouellette B. F., Rapp B. A., Wheeler D. L. 1999; GenBank. Nucleic Acids Res 27:12–17 [CrossRef]
    [Google Scholar]
  3. Connan J., Lacrampe-Couloume G., Magot M. 1996; Origin of gases in reservoirs. In Proceedings of the 1995 Gas Research Conference , vol. 1 pp  21–61 Edited by Dolenc D. A. Rockville, MD: Government Institutes;
    [Google Scholar]
  4. Cord-Ruwisch R. 1985; A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 4:33–36 [CrossRef]
    [Google Scholar]
  5. Davey M. E., Wood W. A., Key R., Nakamura K., Stahl D. A. 1993; Isolation of three species of Geotoga and Petrotoga : two new genera, representing a new lineage in the bacterial line of descent distantly related to the “ Thermotogales ”. Syst Appl Microbiol 16:191–200 [CrossRef]
    [Google Scholar]
  6. Fardeau M.-L., Ollivier B., Patel B. K. C., Magot M., Thomas P., Rimbault A., Rocchiccioli F., Garcia J.-L. 1997; Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. Int J Syst Bacteriol 47:1013–1019 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  8. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  9. Hungate R. E. 1969; A roll-tube method for the cultivation of strict anaerobes. Methods Microbiol 3B:117–132
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–232 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  11. L'Haridon S., Miroshnichenko M. L., Hippe H., Fardeau M. L., Bonch-Osmolovskaya E. A., Stackebrandt E., Jeanthon C. 2002; Petrotoga olearia sp. nov. and Petrotoga sibirica sp. nov., two thermophilic bacteria isolated from a continental petroleum reservoir in Western Siberia. Int J Syst Evol Microbiol 52:1715–1722 [CrossRef]
    [Google Scholar]
  12. Lien T., Madsen M., Rainey F. A., Birkeland N. K. 1998; Petrotoga mobilis sp. nov., from a North Sea oil-production well. Int J Syst Bacteriol 48:1007–1013 [CrossRef]
    [Google Scholar]
  13. Magot M., Ollivier B., Patel B. K. C. 2000; Microbiology of petroleum reservoirs. Antonie van Leeuwenhoek 77:103–116 [CrossRef]
    [Google Scholar]
  14. Maidak B. L., Cole J. R., Lilburn T. G., Parker C. T. Jr, Saxman P. R., Farris R. J., Garrity G. M., Olsen G. J., Schmidt T. M., Tiedje J. M. 2001; The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29:173–174 [CrossRef]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  16. Miranda-Tello E., Fardeau M.-L., Thomas P., Ramirez F., Casalot L., Cayol J.-L., Garcia J.-L., Ollivier B. 2004; Petrotoga mexicana sp. nov., a novel thermophilic, anaerobic and xylanolytic bacterium isolated from an oil-producing well in the Gulf of Mexico. Int J Syst Evol Microbiol 54:169–174 [CrossRef]
    [Google Scholar]
  17. Ollivier B., Cayol J.-L. 2005; The fermentative, iron-reducing, and nitrate-reducing microorganisms. In Petroleum Microbiology pp  71–88 Edited by Ollivier B., Magot M. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  18. Saito N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Stetter K. O., Huber R., Blöchl E., Kurr M., Eden R. D., Fielder M., Cash H., Vance I. 1993; Hyperthermophilic archaea are thriving in deep North Sea and Alaskan oil reservoirs. Nature 365:743–745 [CrossRef]
    [Google Scholar]
  20. Thabet O. B., Fardeau M.-L., Joulian C., Thomas P., Hamdi M., Garcia J.-L., Ollivier B. 2004; Clostridium tunisiense sp. nov., a new proteolytic, sulfur-reducing bacterium isolated from an olive mill wastewater contaminated by phosphogypse. Anaerobe 10:185–190 [CrossRef]
    [Google Scholar]
  21. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
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