A novel thermophilic, moderately halophilic, rod-shaped bacterium, strain MET-BT, with a sheath-like outer structure (toga) was isolated from an offshore oil-producing well in Congo, West Africa. Strain MET-BT was a Gram-negative bacterium with the ability to reduce elemental sulfur, but not sulfate, thiosulfate or sulfite into sulfide. The optimum growth conditions were 60 °C, pH 6.7–7.2 and 4–6 % NaCl. The DNA G+C content was 34.6 mol%. Strain MET-BT was phylogenetically related to members of the genus Petrotoga; Petrotoga miotherma, Petrotoga olearia and Petrotoga mexicana were the closest relatives, with type strains exhibiting more than 99 % identity in an analysis of small-subunit rRNA gene sequences. The values for DNA–DNA relatedness between the type strains of these three species and strain MET-BT were less than 42 %. As MET-BT was found to be genetically and physiologically different from other species of the genus Petrotoga, this strain is proposed as representing a novel species, for which the name Petrotoga halophila sp. nov. is proposed. The type strain is MET-BT (=DSM 16923T=CCUG 50214T).
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