RT Journal Article SR Electronic(1) A1 Miranda-Tello, Elizabeth A1 Fardeau, Marie-Laure A1 Joulian, Catherine A1 Magot, Michel A1 Thomas, Pierre A1 Tholozan, Jean-Luc A1 Ollivier, BernardYR 2007 T1 Petrotoga halophila sp. nov., a thermophilic, moderately halophilic, fermentative bacterium isolated from an offshore oil well in Congo JF International Journal of Systematic and Evolutionary Microbiology, VO 57 IS 1 SP 40 OP 44 DO https://doi.org/10.1099/ijs.0.64516-0 PB Microbiology Society, SN 1466-5034, AB A novel thermophilic, moderately halophilic, rod-shaped bacterium, strain MET-BT, with a sheath-like outer structure (toga) was isolated from an offshore oil-producing well in Congo, West Africa. Strain MET-BT was a Gram-negative bacterium with the ability to reduce elemental sulfur, but not sulfate, thiosulfate or sulfite into sulfide. The optimum growth conditions were 60 °C, pH 6.7–7.2 and 4–6 % NaCl. The DNA G+C content was 34.6 mol%. Strain MET-BT was phylogenetically related to members of the genus Petrotoga; Petrotoga miotherma, Petrotoga olearia and Petrotoga mexicana were the closest relatives, with type strains exhibiting more than 99 % identity in an analysis of small-subunit rRNA gene sequences. The values for DNA–DNA relatedness between the type strains of these three species and strain MET-BT were less than 42 %. As MET-BT was found to be genetically and physiologically different from other species of the genus Petrotoga, this strain is proposed as representing a novel species, for which the name Petrotoga halophila sp. nov. is proposed. The type strain is MET-BT (=DSM 16923T=CCUG 50214T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64516-0