1887

Abstract

The marine bacterial strain HY9 was isolated from sediment from the South China Sea. Strain HY9 is aerobic, heterotrophic and rose-pigmented. The cells are non-motile and curved, i.e. ring-like or horseshoe-shaped. The 16S rRNA gene sequence of strain HY9 was determined and searches revealed that it possessed significant sequence similarities with respect to species (92.8–93.6 %). Phylogenetic analysis confirmed that strain HY9 was tightly clustered with members of the genus . The cellular morphology and chemotaxonomic and phenotypic properties of strain HY9 showed that it should be classified as a member of the genus . Significant evolutionary distances and a range of phenotypic features distinguished strain HY9 from previously described species. Hence, strain HY9 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is HY9 (=CGMCC 1.6102=JCM 14011). On the basis of this study and previously described properties of species, an emended description of the genus is proposed.

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2006-12-01
2019-10-18
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References

  1. Bowman, J. P., Nichols, C. M. & Gibson, J. A. E. ( 2003; ). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53, 1343–1355.[CrossRef]
    [Google Scholar]
  2. Brettar, I., Christen, R. & Höfle, M. G. ( 2004a; ). Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the Cytophaga–Flavobacterium–Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54, 65–70.[CrossRef]
    [Google Scholar]
  3. Brettar, I., Christen, R. & Höfle, M. G. ( 2004b; ). Aquiflexum balticum gen. nov., sp. nov., a novel marine bacterium of the Cytophaga–Flavobacterium–Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54, 2335–2341.[CrossRef]
    [Google Scholar]
  4. Cho, J. C. & Giovannoni, S. J. ( 2003; ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria. Int J Syst Evol Microbiol 53, 1031–1036.[CrossRef]
    [Google Scholar]
  5. Dong, X.-Z. & Cai, M.-Y. ( 2001; ). Determinative Manual for Routine Bacteriology. Beijing: Scientific Press.
  6. Euzéby, J. P. ( 1998; ). Taxonomic note: necessary correction of specific and subspecific epithets according to Rules 12c and 13b of the International Code of Nomenclature of Bacteria (1990 Revision). Int J Syst Bacteriol 48, 1073–1075.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1997; ). An alternative least-squares approach to inferring phylogenies from pairwise distances. Syst Biol 46, 101–111.[CrossRef]
    [Google Scholar]
  8. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum changes for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  9. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  10. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  11. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  12. Nedashkovskaya, O. L., Kim, S. B., Lee, M. S., Park, M. S., Lee, K. H., Lysenko, A. M., Oh, H. W., Mikhailov, V. V. & Bae, K. S. ( 2005; ). Cyclobacterium amurskyense sp. nov., a novel marine bacterium isolated from sea water. Int J Syst Evol Microbiol 55, 2391–2394.[CrossRef]
    [Google Scholar]
  13. Raj, H. D. & Maloy, S. R. ( 1990; ). Proposal of Cyclobacterium marinum gen. nov., comb. nov. for a marine bacterium previously assigned to the genus Flectobacillus. Int J Syst Bacteriol 40, 337–347.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Seidler, R. J. & Mandel, M. ( 1971; ). Quantitative aspects of deoxyribonucleic acid renaturation: base composition, state of chromosome replication, and polynucleotide homologies. J Bacteriol 106, 608–614.
    [Google Scholar]
  16. Zhang, D., Yang, H., Huang, Z., Zhang, W. & Liu, S.-J. ( 2002; ). Rhodopseudomonas faecalis sp. nov., a phototrophic bacterium isolated from an anaerobic reactor that digests chicken faeces. Int J Syst Evol Microbiol 52, 2055–2060.[CrossRef]
    [Google Scholar]
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Morphology of HY9 visualized with transmission electron microscopy. Bar, 2 µm.

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Phylogenetic trees, constructed with the maximum-parsimony (a) and minimum-evolution (b) methods, showing that strain HY9 ( sp. nov.) is consistently clustered with and . [PDF](18 KB)

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Complete fatty acid compositions (%) of the type strains of the three species. [PDF](106 KB)

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